Evento
FTIR and Raman characterization of DMPC lipid vesicles with different biomolecules
Corrales Chahar, Fernanda; Lacosegliaz, Mariano José
; Sanini, M. V.; Diaz, Silvia Beatriz; Ben Altabef, Aida
; Gervasi, Claudio; Álvarez, P.
Tipo del evento:
Congreso
Nombre del evento:
III Latin American Federation of Biophysical Societies; IX IberoAmerican Congress of Biophysics; XLV Reunión Anual Sociedad Argentina de Biofísica
Fecha del evento:
23/11/2016
Institución Organizadora:
Sociedad Argentina de Biofísica;
Título del Libro:
Resumenes de la III Latin American Federation of Biophysical Societies, IX IberoAmerican Congress of Biophysics y de la XLV Reunión Anual Sociedad Argentina de Biofísica
Editorial:
Sociedad Argentina de Biofísica
Idioma:
Inglés
Clasificación temática:
Resumen
This work aims at gaining insight into the characteristics of the membrane-biomolecule interaction in order to understand the action mechanisms of the biomolecules, their effectivity and physicochemical properties. Vitamin C (ascorbic acid) is an essential cofactor for certain enzimes that are widely used in medicine and in food industry, annonacin is a mono-THF acetogenin a neurotoxic chemical compound found in some fruits with potential applicability as insecticide, acaricide, fungicide, as well as antiparasitic and antitumor agents, finally, valproic acid is an antiepileptic drug and mood stabilizer with action on different channels of the central nervous system. 1,2-Dimyristoyl-sn-glycero-3-phosphocholine (DMPC) multilamellar vesicles were prepared in the presence of different concentrations of the above-mentioned biomolecules. FTIR and Raman spectroscopies were used to analyse the participation of specific functional groups in the biomolecule-membrane interaction. Spectra recorded for the complexes Biomolecule:DMPC were analysed in comparison with those of the pure liquid, studying spectral bands related to the inner regions of the lipid bilayer (hydrophobic regions) and the interphasial region (hydrophilic region). Low and high-frequency shifts were observed depending on the group and the temperature. The effect of each biomolecule on the transition temperature (Tm) the phase gel transition (Lβ) – liquid crystalline (Lα) of the phospholipids exhibits a feature transition. Raman spectra show variations according to the presence of ascorbica acid, valproic acid or annonacin.
Palabras clave:
DMPC lipid vesicles
,
Biomolecules
,
FTIR
,
Raman
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Eventos(INQUINOA)
Eventos de INST.DE QUIMICA DEL NOROESTE
Eventos de INST.DE QUIMICA DEL NOROESTE
Citación
FTIR and Raman characterization of DMPC lipid vesicles with different biomolecules; III Latin American Federation of Biophysical Societies; IX IberoAmerican Congress of Biophysics; XLV Reunión Anual Sociedad Argentina de Biofísica; San Miguel de Tucumán; Argentina; 2016; 358-360
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