Artículo
Electrochemical microfluidic immunosensor with graphene-decorated gold nanoporous for T-2 mycotoxin detection
Fernandez Solis, Laura N.; Silva Junior, Gilberto J.; Bertotti, Mauro; Angnes, Lúcio; Pereira, Sirley Vanesa
; Fernández Baldo, Martín Alejandro
; Regiart, Daniel Matias Gaston



Fecha de publicación:
06/2024
Editorial:
Elsevier Science
Revista:
Talanta
ISSN:
0039-9140
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
T-2 is one of the most potent cytotoxic food-borne mycotoxins. In this work, we have developed and characterized an electrochemical microfluidic immunosensor for T-2 toxin quantification in wheat germ samples. T-2 toxin detection was carried out using a competitive immunoassay method based on monoclonal anti-T-2 antibodies immobilized on the poly(methyl methacrylate) (PMMA) microfluidic central channel. The platinum wire working electrode at the end of the channel was in situ modified by a single-step electrodeposition procedure with reduced graphene oxide (rGO)-nanoporous gold (NPG). T-2 toxin in the sample was allowed to compete with T-2-horseradish peroxidase (HRP) conjugated for the specific recognizing sites of immobilized anti-T-2 monoclonal antibodies. The HRP, in the presence of hydrogen peroxide (H2O2), catalyzes the oxidation of 4-tert-butylcatechol (4-TBC), whose back electrochemical reduction was detected on the nanostructured electrode at − 0.15 V. Thus, at low T-2 concentrations in the sample, more enzymatically conjugated T-2 will bind to the capture antibodies, and, therefore, a higher current is expected. The detection limits found for electrochemical immunosensor, and commercial ELISA procedure were 0.10 μg kg− 1 and 10 μg kg− 1, and the intra- and inter-assay coefficients of variation were below 5.35% and 6.87%, respectively. Finally, our microfluidic immunosensor to T-2 toxin will significantly contribute to faster, direct, and secure in situ analysis in agricultural samples.
Palabras clave:
MICROFLUIDIC INMUNOSENSOR
,
ELECTROCHEMICAL
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Identificadores
Colecciones
Articulos(INQUISAL)
Articulos de INST. DE QUIMICA DE SAN LUIS
Articulos de INST. DE QUIMICA DE SAN LUIS
Citación
Fernandez Solis, Laura N.; Silva Junior, Gilberto J.; Bertotti, Mauro; Angnes, Lúcio; Pereira, Sirley Vanesa; et al.; Electrochemical microfluidic immunosensor with graphene-decorated gold nanoporous for T-2 mycotoxin detection; Elsevier Science; Talanta; 273; 6-2024; 1-8
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