Artículo
SpoIIIE mechanism of directional translocation involves target search coupled to sequence-dependent motor stimulation
Cattoni, Diego Ignacio
; Chara, Osvaldo
; Godefroy, Cédric; Margeat, Emmanuel; Trigueros, Sonia; Milhiet, Pierre Emmanuel; Nöllmann, Marcelo
Fecha de publicación:
04/2013
Editorial:
Embo Press
Revista:
Embo Reports
ISSN:
1469-221X
e-ISSN:
1469-3178
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
SpoIIIE/FtsK are membrane‐anchored, ATP‐fuelled, directional motors responsible for chromosomal segregation in bacteria. Directionality in these motors is governed by interactions between specialized sequence‐recognition modules (SpoIIIE‐γ/FtsK‐γ) and highly skewed chromosomal sequences (SRS/KOPS). Using a new combination of ensemble and single‐molecule methods, we dissect the series of steps required for SRS localization and motor activation. First, we demonstrate that SpoIIIE/DNA association kinetics are sequence independent, with binding specificity being uniquely determined by dissociation. Next, we show by single‐molecule and modelling methods that hexameric SpoIIIE binds DNA non‐specifically and finds SRS by an ATP‐independent target search mechanism, with ensuing oligomerization and binding of SpoIIIE‐γ to SRS triggering motor stimulation. Finally, we propose a new model that provides an entirely new interpretation of previous observations for the origin of SRS/KOPS‐directed translocation by SpoIIIE/FtsK.
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Articulos(IFLYSIB)
Articulos de INST.FISICA DE LIQUIDOS Y SIST.BIOLOGICOS (I)
Articulos de INST.FISICA DE LIQUIDOS Y SIST.BIOLOGICOS (I)
Citación
Cattoni, Diego Ignacio; Chara, Osvaldo; Godefroy, Cédric; Margeat, Emmanuel; Trigueros, Sonia; et al.; SpoIIIE mechanism of directional translocation involves target search coupled to sequence-dependent motor stimulation; Embo Press; Embo Reports; 14; 5; 4-2013; 473-479
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