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dc.contributor.author
Carabajal, Monica Patricia Antonella
dc.contributor.author
Bonacina, Julieta
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Scarinci, María Noelia
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Albarracín, Virginia Helena
dc.contributor.author
Cantero, Maria del Rocio
dc.contributor.author
Cantiello, Horacio Fabio
dc.date.available
2024-05-17T10:24:23Z
dc.date.issued
2023-10
dc.identifier.citation
Carabajal, Monica Patricia Antonella; Bonacina, Julieta; Scarinci, María Noelia; Albarracín, Virginia Helena; Cantero, Maria del Rocio; et al.; The bacterial tubulin homolog FtsZ generates electrical oscillations; Elsevier; Biochemical and Biophysical Research Communications; 687; 10-2023; 1-10
dc.identifier.issn
1090-2104
dc.identifier.uri
http://hdl.handle.net/11336/235555
dc.description.abstract
FtsZ, a major cytoskeletal protein in all bacteria and archaea, forms a ring that directs cytokinesis. Bacterial FtsZis considered the ancestral homolog of the eukaryotic microtubule (MT)-forming tubulins, sharing GTPase activityand the ability to assemble into protofilaments, rings, and sheets, but not MTs. Previous studies from ourlaboratory demonstrated that structures of isolated brain MTs spontaneously generate electrical oscillations andbursts of electrical activity similar to action potentials. No information about whether the prokaryotic tubulinsmay share similar properties is available. Here, we obtained by ammonium sulfate precipitation an enrichedprotein fraction of the endogenous FtsZ from wild-type Escherichia coli ATCC 25922 without any transfection oroverexpression of the protein. As revealed by electron microscopy, FtsZ was detected by dot blot analysis andimmunofluorescence that assembled into filaments and sheets in a polymerization buffer. We used the patchclamptechnique to explore the electrical properties of sheets of FtsZ and bacterial cells. Electrical recordingsat various holding potentials ranging from ±200 mV showed a complex oscillatory behavior, with several peakfrequencies between 12 and 110 Hz in the power spectra and a linear mean current response. To confirm theoscillatory electrical behavior of FtsZ we also conducted experiments with commercial recombinant FtsZ, withsimilar results. We also detected, by local field potentials, similar electrical oscillations in K+-depolarized pelletsof E. coli cultures. FtsZ oscillations had a wider range of frequency peaks than MT sheets from eukaryotic origin.The findings indicate that the bacterial cytoskeleton generates electrical oscillators that may play a relevant rolein cell division and unknown signaling mechanisms in bacterial populations.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
FtsZ
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Prokaryote tubulins
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Microtubules
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Patch clamp
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Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
The bacterial tubulin homolog FtsZ generates electrical oscillations
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-05-14T13:46:05Z
dc.journal.volume
687
dc.journal.pagination
1-10
dc.journal.pais
Países Bajos
dc.description.fil
Fil: Carabajal, Monica Patricia Antonella. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet Noa Sur. Instituto Multidisciplinario de Salud, Tecnologia y Desarrollo. - Universidad Nacional de Santiago del Estero. Instituto Multidisciplinario de Salud, Tecnologia y Desarrollo.; Argentina
dc.description.fil
Fil: Bonacina, Julieta. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet Noa Sur. Instituto Multidisciplinario de Salud, Tecnologia y Desarrollo. - Universidad Nacional de Santiago del Estero. Instituto Multidisciplinario de Salud, Tecnologia y Desarrollo.; Argentina
dc.description.fil
Fil: Scarinci, María Noelia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet Noa Sur. Instituto Multidisciplinario de Salud, Tecnologia y Desarrollo. - Universidad Nacional de Santiago del Estero. Instituto Multidisciplinario de Salud, Tecnologia y Desarrollo.; Argentina
dc.description.fil
Fil: Albarracín, Virginia Helena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Cantero, Maria del Rocio. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet Noa Sur. Instituto Multidisciplinario de Salud, Tecnologia y Desarrollo. - Universidad Nacional de Santiago del Estero. Instituto Multidisciplinario de Salud, Tecnologia y Desarrollo.; Argentina
dc.description.fil
Fil: Cantiello, Horacio Fabio. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet Noa Sur. Instituto Multidisciplinario de Salud, Tecnologia y Desarrollo. - Universidad Nacional de Santiago del Estero. Instituto Multidisciplinario de Salud, Tecnologia y Desarrollo.; Argentina
dc.journal.title
Biochemical and Biophysical Research Communications
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.bbrc.2023.149186
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