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dc.contributor.author
Andersen, Natalia Denise
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dc.contributor.author
Kuo, Blanche
dc.contributor.author
Piñero, Gonzalo Miguel
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dc.contributor.author
Ravelo, Kristine
dc.contributor.author
Rai, Priyamvada
dc.contributor.author
Monje, Paula
dc.date.available
2024-05-15T11:59:20Z
dc.date.issued
2016
dc.identifier.citation
Human Schwann cell senescence is not prevented by ectopic expression of human telomerase reverse transcriptase; Society for Neuroscience 46th Annual Meeting; San Diego; Estados Unidos; 2016; 1-1
dc.identifier.uri
http://hdl.handle.net/11336/235392
dc.description.abstract
Isolated human Schwann cells (hSCs) typically become senescent and unresponsive to mitogenic factors with continued in vitro expansion. Data from our lab has shown that adult nerve-derived hSCs, in contrast to rodent (rat) SCs, inevitably stop proliferating and acquire a senescent phenotype characterized by high levels of senescence-associated (SA)-β galactosidase activity and morphological changes that include cell enlargement and appearance of multinucleated cells. As opposed to rodent SCs, hSC cultures consist of mixed populations of proliferating cells, senescent cells and cells at different stages of differentiation regardless of the nerve of origin and other donor-specific factors. RNA-seq analysis of representative cultures of hSCs did not reveal the presence of telomerase reverse transcriptase (TERT) mRNA while other TERT-related genes (e.g. TERF1, telomeric repeat binding factor, and TEP1, telomeraseassociated protein) were well-represented in the hSC transcriptome. In an attempt to overcome senescence, we used retroviral vectors and antibiotic selection to generate hSC lines ectopically expressing human (h)-TERT. For these experiments, highly proliferative, non-senescent, early passage hSC cultures were stably transduced with the retroviruses h-TERT-hygro or h-TERTpuro, each encoding the h-TERT gene along with hygromycin or puromycin resistance genes, respectively. Transduced hSC cultures from three different donors were selected and subjected to three rounds of expansion in medium containing chemical mitogens. Subsequently, the cultures were analyzed for their rate of proliferation by means of EdU incorporation assays and the acquisition of senescence by means of SA-β galactosidase activity assays in each round. We found that whereas ectopic h-TERT expression extended the lifespan of cultured hSCs when compared to non-infected or GFP-expressing cells, it was not sufficient to confer immortalization and overcome senescence. In sum, our results suggest that progression of the hSCs to a senescent state likely is stress-induced rather than dependent on replication-associated telomere shortening
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Society for Neuroscience
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
SCHWANN CELLS
dc.subject
SENESCENCE
dc.subject
PROLIFERATION
dc.subject
RETROVIRAL VECTORS
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Human Schwann cell senescence is not prevented by ectopic expression of human telomerase reverse transcriptase
dc.type
info:eu-repo/semantics/publishedVersion
dc.type
info:eu-repo/semantics/conferenceObject
dc.type
info:ar-repo/semantics/documento de conferencia
dc.date.updated
2024-04-24T13:46:28Z
dc.journal.pagination
1-1
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
San Diego
dc.description.fil
Fil: Andersen, Natalia Denise. Miami University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Kuo, Blanche. Miami University; Estados Unidos
dc.description.fil
Fil: Piñero, Gonzalo Miguel. Miami University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Ravelo, Kristine. Miami University; Estados Unidos
dc.description.fil
Fil: Rai, Priyamvada. Miami University. School Of Medicine; Estados Unidos
dc.description.fil
Fil: Monje, Paula. Miami University. School Of Medicine; Estados Unidos
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sfn.org/Annual-Meeting/Neuroscience-2016/Sessions-and-Events/Program/~/media/SfN/Documents/Annual%20Meeting/FinalProgram/NS2016/Full%20Abstract%20PDFs%202016/SfN16_Abstract%20PDFs%20%20Posters_3_Mon_PM.ashx
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sfn.org/meetings/past-and-future-annual-meetings
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Autor
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Autor
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Autor
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dc.coverage
Internacional
dc.type.subtype
Congreso
dc.description.nombreEvento
Society for Neuroscience 46th Annual Meeting
dc.date.evento
2016-11-12
dc.description.ciudadEvento
San Diego
dc.description.paisEvento
Estados Unidos
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dc.type.publicacion
Book
dc.description.institucionOrganizadora
Society for Neuroscience
dc.source.libro
Programa: Neuroscience 2016
dc.date.eventoHasta
2016-11-16
dc.type
Congreso
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