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dc.contributor.author
Marani, Mariela Mirta
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dc.date.available
2024-05-15T10:23:51Z
dc.date.issued
2022
dc.identifier.citation
Prospection of bioactive peptides from South America anuran species; 36th European Peptide Symposium and the 12th International Peptide Symposium; Sitges; España; 2022; 1-29
dc.identifier.uri
http://hdl.handle.net/11336/235381
dc.description.abstract
The secretion of multiple antimicrobial peptides (AMPs) from the skin allows amphibians to occupy moist environments with a high density of microorganisms. Although there are some limitations to their pharmacological applicability, AMPs are suitable candidates for developing new antibiotics, either directly or as templates. The main focus of our BIAPEP research group is the prospecting of peptides of technological interest through the exploration of anurans of Argentine biodiversity. Our current objective is the functional and structural evaluation of antimicrobial peptides present in the skin of amphibians together with the design of improved-performance analogs. To this end, we combined HPLC-MS/MS, molecular biology, and microbiology for peptide identification. We described more than 70 mature host defense peptides from twelve anuran species (Pleurodema thaul, P. bufoninum, P. somuncurence, Odonthophrynus occidentalis, Boana pulchella, B. punctata, B. prasina, Leptodactylus latrans, L. chaquensis, L. pustulatus, Physalaemus albonotatus, Pseudopaludicola boliviana). Identified peptides had between 4 and 69 residues, molecular weights ranged from 892 to 6,022 Da, and net charges from -8 to +4. In silico and structural studies of several peptides were performed. A selected set of these peptides was chemically synthesized, characterized, and tested for their bioactivity. We observed antimicrobial (MIC ≥14µM) and antiparasitic (IC50 ≥10µM) peptides. Additionally, some peptides showed an ability to scavenge radicals in vitro in different systems showing potential antioxidant capacity. The compilation, comparison, and analysis of all identified sequences demonstrate the hypervariability of peptide sequences in the studied species and the relevance of prospection of unexplored biodiversity.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
John Wiley & Sons Ltd
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dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ANFIBIOS
dc.subject
ANTIMICROBIANOS
dc.subject
PEPTIDOS
dc.subject
BIOPROSPECCIÓN
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Prospection of bioactive peptides from South America anuran species
dc.type
info:eu-repo/semantics/publishedVersion
dc.type
info:eu-repo/semantics/conferenceObject
dc.type
info:ar-repo/semantics/documento de conferencia
dc.date.updated
2023-11-13T15:54:14Z
dc.journal.pagination
1-29
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Chichester
dc.description.fil
Fil: Marani, Mariela Mirta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; Argentina
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.eurpepsoc.com/proceedings-of-the-36th-european-and-the-12th-international-peptide-symposium-in-sitges-spain-2022-are-now-available/
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Autor
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Autor
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Autor
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dc.coverage
Internacional
dc.type.subtype
Simposio
dc.description.nombreEvento
36th European Peptide Symposium and the 12th International Peptide Symposium
dc.date.evento
2022-08-28
dc.description.ciudadEvento
Sitges
dc.description.paisEvento
España
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dc.type.publicacion
Book
dc.description.institucionOrganizadora
European Peptide Society
dc.source.libro
Proceedings of the 36th European Peptide Symposium and the 12th International Peptide Symposium
dc.date.eventoHasta
2022-09-02
dc.type
Simposio
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