Evento
GABA transporters in human lymphocytes
Tipo del evento:
Congreso
Nombre del evento:
XXVIII Congreso Anual De La Sociedad Argentina De Investigación En Neurociencias y Reunión satélite sobre Neurobiología del Comportamiento: “NeuroetologÍa y Neurobiología de la Memoria en el cono sur” Un homenaje a Héctor Maldonado
Fecha del evento:
30/09/2013
Institución Organizadora:
Sociedad Argentina de Investigación en Neurociencias;
Título del Libro:
XXVIII Congreso Anual De La Sociedad Argentina De Investigación En Neurociencias y Reunión satélite sobre Neurobiología del Comportamiento: “NeuroetologÍa y Neurobiología de la Memoria en el cono sur” Un homenaje a Héctor Maldonado
Editorial:
Sociedad Argentina de Investigación en Neurociencias
Idioma:
Inglés
Clasificación temática:
Resumen
GABA is the main inhibitory neurotransmitter in CNS and is associated to several neurological disorders. GABA transporters (GATs) play a critical role in GABA level regulation by allowing re-uptake of neuronal secreted GABA. Four GAT subtypes (GAT 1-3 and BGT-1) have been described in humans. Previously, we reported a complete GABAergic system in lymphocytes. Here, we studied the modulation of the expression and activity of GATs in human lymphocytes by the mitogen phytohemagglutinin (PHA). We determined mRNA GAT expression in activated and resting cells (with and without PHA, respectively). GAT 3 was not detected under any condition, whereas GAT 2 and BGT-1 were detected in all activated cells. Expression of GAT 1 was variable among samples and conditions. In line with these observations, incubation with PHA also increased [3H]GABA uptake. To evaluate the physiological role of GATs we determined cell proliferation by PHA in the presence of nipecotic acid (NA), a GAT inhibitor. Cell proliferation was negatively modulated by NA. In addition, secreted GABA was detected only in supernatant from activated lymphocyte cultures. Taken together, our results show that lymphocytes express functional GATs whose expression is modulated by PHA, GATs regulate cell proliferation, and lymphocyte cells have the ability to secrete GABA. Pharmacological modulation of GATs present in lymphocytes could be a new target to modulate immune response.
Palabras clave:
GABA TRANSPORTERS
,
LYMPHOCYTES
,
GABA
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Colecciones
Eventos(INIBIBB)
Eventos de INST.DE INVEST.BIOQUIMICAS BAHIA BLANCA (I)
Eventos de INST.DE INVEST.BIOQUIMICAS BAHIA BLANCA (I)
Citación
GABA transporters in human lymphocytes; XXVIII Congreso Anual De La Sociedad Argentina De Investigación En Neurociencias y Reunión satélite sobre Neurobiología del Comportamiento: “NeuroetologÍa y Neurobiología de la Memoria en el cono sur” Un homenaje a Héctor Maldonado; Argentina; 2013; 311-311
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