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dc.contributor.author Ronchetti, Sonia Alejandra
dc.contributor.author Bianchi, Maria Silvia
dc.contributor.author Duvilanski, Beatriz Haydee
dc.contributor.author Cabilla, Jimena Paula
dc.date.available 2017-09-01T18:14:12Z
dc.date.issued 2016-04
dc.identifier.citation Ronchetti, Sonia Alejandra; Bianchi, Maria Silvia; Duvilanski, Beatriz Haydee; Cabilla, Jimena Paula; In vivo and in vitro arsenic exposition induces oxidative stress in anterior pituitary gland; Sage Publications; International Journal Of Toxicology; 35; 4; 4-2016; 463-475
dc.identifier.issn 1091-5818
dc.identifier.uri http://hdl.handle.net/11336/23454
dc.description.abstract Inorganic arsenic (iAs) is at the top of toxic metalloids. Inorganic arsenic-contaminated water consumption is one of the greatest environmental health threats worldwide. Human iAs exposure has been associated with cancers of several organs, neurological disorders, and reproductive problems. Nevertheless, there are no reports describing how iAs affects the anterior pituitary gland. The aim of this study was to investigate the mechanisms involved in iAs-mediated anterior pituitary toxicity both in vivo and in vitro. We showed that iAs administration (from 5 to 100 ppm) to male rats through drinking water increased messenger RNA expression of several oxidative stress-responsive genes in the anterior pituitary gland. Serum prolactin levels diminished, whereasluteinizing hormone (LH) levels were only affected at the higher dose tested. In anterior pituitary cells in culture, 25 micromol/L iAssignificantly decreased prolactin release in a time-dependent fashion, whereas LH levels remained unaltered. Cell viability was significantly reduced mainly by apoptosis evidenced by morphological and phosphatidylserine externalization studies. This process is characterized by early depolarization of mitochondrial membrane potential and increased levels of reactive oxygen species. Expression of some key oxidative stress-responsive genes, such as heme oxygenase-1 and metallothionein-1, was also stimulated by iAs exposure. The antioxidant N-acetyl cysteine prevented iAs-induced effects on the expression of oxidative stress markers, prolactin release, and apoptosis. In summary, the present work demonstrates for the first time that iAs reduces prolactin releaseboth in vivo and in vitro and induces apoptosis in anterior pituitary cells, possibly resulting from imbalanced cellular redox status.
dc.format application/pdf
dc.language.iso eng
dc.publisher Sage Publications
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject ARSENIC
dc.subject OXIDATIVE STRESS
dc.subject APOPTOSIS
dc.subject ANTERIOR PITUITARY CELLS
dc.subject.classification Bioquímica y Biología Molecular
dc.subject.classification Medicina Básica
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.title In vivo and in vitro arsenic exposition induces oxidative stress in anterior pituitary gland
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-08-24T15:32:40Z
dc.identifier.eissn 1092-874X
dc.journal.volume 35
dc.journal.number 4
dc.journal.pagination 463-475
dc.journal.pais Estados Unidos
dc.description.fil Fil: Ronchetti, Sonia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
dc.description.fil Fil: Bianchi, Maria Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil Fil: Duvilanski, Beatriz Haydee. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
dc.description.fil Fil: Cabilla, Jimena Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
dc.journal.title International Journal Of Toxicology
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/abs/10.1177/1091581816645797
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1177/1091581816645797
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/pmid/27151894


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)