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dc.date.available
2024-05-06T13:19:40Z
dc.identifier.citation
Bontempi, Iván; Arias, Diego Gustavo; Marcipar, Iván Sergio; (2024): Improved Serodiagnosis of Trypanosoma vivax Infections in Cattle Reveals High Infection Rates in the Livestock Regions of Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. (dataset). http://hdl.handle.net/11336/234547
dc.identifier.uri
http://hdl.handle.net/11336/234547
dc.description.abstract
Bovine trypanosomosis, caused by Trypanosoma vivax, currently affects cattle and has a significant economic impact in sub-Saharan Africa and South America. The development of new diagnostic antigens is essential to improve and refine existing methods. Our study evaluated the efficacy of two recombinant antigens in detecting specific antibodies in cattle. These antigens are derivatives of an invariant surface glycoprotein (ISG) from T. vivax. A fraction of a previously described antigen (TvY486_0045500), designated TvISGAf, from an African strain was evaluated, and a new ISG antigen from an American isolate, TvISGAm, was identified. The two antigens were expressed as fusion proteins in Escherichia coli: TvISGAf was fused to the MBP-His-tag, and TvISGAm was obtained as a His-tag fused protein. An ELISA evaluation was conducted using these antigens on 149 positive and 63 negative bovine samples. The diagnostic performance was enhanced by the use of a combination of both antigens (referred to as TvISG-based ELISA), achieving a sensitivity of 89.6% and specificity of 93.8%. Following the validation of the TvISG-based ELISA, the seroprevalence of T. vivax infection in 892 field samples from cattle in the central region of Argentina was determined. The mean seroprevalence of T. vivax was 53%, with variation ranging from 21% to 69% among the six departments studied. These results support the use of the TvISG ELISA as a valuable serological tool for the detection and monitoring of T. vivax infection in cattle. Furthermore, we report for the first time the seroprevalence of T. vivax in Argentina, which highlights the widespread endemic nature of the disease in the region. In order to effectively manage the increasing spread of T. vivax in the vast livestock production areas of South America, it is essential to implement consistent surveillance programs and to adopt preventive strategies.
dc.rights
info:eu-repo/semantics/embargoedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.title
Improved Serodiagnosis of Trypanosoma vivax Infections in Cattle Reveals High Infection Rates in the Livestock Regions of Argentina
dc.type
dataset
dc.date.updated
2024-04-30T13:16:57Z
dc.description.fil
Fil: Bontempi, Iván. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Tecnología Inmunológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Arias, Diego Gustavo. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Enzimología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Marcipar, Iván Sergio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Tecnología Inmunológica; Argentina
dc.rights.embargoDate
2024-06-01
dc.datacite.PublicationYear
2024
dc.datacite.Creator
Bontempi, Iván
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dc.datacite.Creator
Arias, Diego Gustavo
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dc.datacite.Creator
Marcipar, Iván Sergio
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dc.datacite.affiliation
Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Tecnología Inmunológica
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dc.datacite.affiliation
Consejo Nacional de Investigaciones Científicas y Técnicas
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dc.datacite.affiliation
Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Enzimología Molecular
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dc.datacite.affiliation
Consejo Nacional de Investigaciones Científicas y Técnicas
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dc.datacite.affiliation
Consejo Nacional de Investigaciones Científicas y Técnicas
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dc.datacite.affiliation
Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Tecnología Inmunológica
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dc.datacite.publisher
Consejo Nacional de Investigaciones Científicas y Técnicas
dc.datacite.subject
Otras Ciencias Veterinarias
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dc.datacite.subject
Ciencias Veterinarias
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dc.datacite.subject
CIENCIAS AGRÍCOLAS
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dc.datacite.date
02/01/2021-05/01/2023
dc.datacite.DateType
Recolectado
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dc.datacite.language
eng
dc.datacite.version
1.0
dc.datacite.description
resumir: Immunodetection experiments were adapted from a previously described method for T. cruzi (31). Briefly, polyclonal antibodies against TvISGAf and TvISGAm were obtained by immunizing mice with 10 µg of purified recombinant protein at two-week intervals, as previously described (32). T. vivax cells of the Y486 strain, obtained from mice infected for four days, were pelleted and washed twice for 15 minutes at room temperature with sterile PBS buffer and then fixed in 4% (v/v) formaldehyde. After washing, they were permeabilized and blocked for 30 minutes in a medium containing PBS with the addition of 0.1% (v/v) Triton X-100 and 3% (w/v) BSA. The primary antibodies used were either mouse anti-TvISAf sera (1/100) or mouse anti-TvISGAm sera (1/100), along with rabbit anti-TccPx polyclonal antibodies (1/100 dilution). Slide wells were washed three times with blocking solution (3x50 µl). Secondary antibodies diluted 1/100 in blocking buffer (20 µl) were added to the slide wells and incubated for 60 minutes. FITC-conjugated goat anti-rabbit or Alexa 680-conjugated goat anti-mouse antibodies were used as secondary antibodies. DAPI (20 µl/slide well), diluted in PBS (final concentration 0.5 µg/ml), was added to each slide well. Slides were examined with a confocal microscope (Leica).
dc.datacite.DescriptionType
Métodos
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dc.datacite.FundingReference
PICT-2020-SERIEA-025 92
dc.datacite.FunderName
Ministerio de Ciencia, Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica. Fondo para la Investigación Científica y Tecnológica
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dc.subject.keyword
T. vivax seroprevalence
dc.subject.keyword
Dairy cattle
dc.subject.keyword
Endemic disease
dc.subject.keyword
Parasite spreading
dc.datacite.resourceTypeGeneral
dataset
dc.conicet.datoinvestigacionid
15254
dc.datacite.awardTitle
DESARROLLO DE UNA INMUNOCROMATOGRAFÍA DE FLUJO LATERAL PARA EL DIAGNÓSTICO RÁPIDO DE TRYPANOSOMIASIS BOVINA CARÁTULA DE PROYECTO
dc.datacite.geolocation
Santa Fe
dc.datacite.geolocation
Córdoba
dc.datacite.formatedDate
2021-2023
Archivos del conjunto de datos
Archivo
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Table_1__Elisa_diagnostic_results_from_field_tests_.xlsx
Embargado hasta 01-06-2024
54.23Kb
Figure_5.tif
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765.9Kb
Fig_3__TvISGAf-cPX.lif
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204.8Mb
Figure_4.tif
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1.483Mb
table_1.tif
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117.8Kb
Fig_4_Comparison_reactivity_of_TvISGAf__yellow__or_TvISGAm__orange__antigens_or_their_mixture__TvISG__purple_.pzf
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770.4Kb
Figure_3.tif
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8.368Mb
Fig_5__Comparison_of_the_ROC_curves_of_the_anti-IgG_ELISA.pzf
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1.651Mb
Fig_3__TvISGAm-cPX.lif
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96.36Mb
Fig_3_negative.lif
Embargado hasta 01-06-2024
12.26Mb