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dc.contributor.author
Nunes, M. M.
dc.contributor.author
Morrell, J. M.
dc.contributor.author
Santos, F. C. C.
dc.contributor.author
Miragaya, M. H.
dc.contributor.author
Gallelli, Maria Florencia
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dc.contributor.author
Rodriguez, Marcelo
dc.contributor.author
Guimaraes, J. D.
dc.date.available
2024-04-26T14:48:18Z
dc.date.issued
2024-03
dc.identifier.citation
Nunes, M. M.; Morrell, J. M.; Santos, F. C. C.; Miragaya, M. H.; Gallelli, Maria Florencia; et al.; Effect of storage and single layer centrifugation before cryopreservation on stallion sperm cryosurvival; Elsevier Science Inc.; Journal of Equine Veterinary Science; 135; 3-2024; 1-5
dc.identifier.issn
0737-0806
dc.identifier.uri
http://hdl.handle.net/11336/234091
dc.description.abstract
The objectives of this study were to evaluate the effect of a short, cooled storage before cryopreservation onsperm progressive motility (PM) and compare the effect of different centrifugation methods on post-thaw PM ofstored samples. Semen was diluted in chilling extender and aliquoted in 6 protocols: i) Standard centrifugation(SC) followed by freezing; ii) Single Layer Centrifugation (SLC) followed by freezing; iii) Storage for 8 h/5 ◦Cbefore SC; iv) Storage for 8 h/5 ◦C before SLC; v) Storage for 8 h/15 ◦C before SC; and vi) Storage for 8 h/15 ◦Cbefore SLC. PM was assessed before centrifugation, after centrifugation, and post-thawing. Stallions were classifiedas “good freezers” (GF) or “bad freezers” (BF). The PM in samples immediately frozen was greater than inthe stored ones (71.98 ± 14.2, 52.91 ± 17.8, 53.93 ± 18.9 for no storage, 5 ºC storage and 15 ºC storage,respectively) (P˂ 0.0001). There was an effect of storage condition (p ˂ 0.0001), centrifugation method (p ˂0.0001), and freezability (P=0.0016), with an interaction between them (P= 0.0004), on PM after centrifugation.Post-thaw PM was greater in samples treated by SLC than in samples processed by SC, for all storageconditions (p ˂ 0.05). All BF stallions ‘showed post-thaw PM ˂ 30 % when samples were previously stored.Storage at 5 ºC or 15º C for 8 h maintains an appropriate quality in GF stallions. Applying a sperm selectiontechnique as SLC is suggested to improve post-thaw motility, allowing GF straws to be frozen after storage,although BF semen should be prepared by SLC immediately after collection.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science Inc.
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dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CRYOPRESERVATION
dc.subject
EXTENDER
dc.subject
SINGLE LAYER CENTRIFUGATION
dc.subject.classification
Ciencias Veterinarias
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dc.subject.classification
Ciencias Veterinarias
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dc.subject.classification
CIENCIAS AGRÍCOLAS
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dc.title
Effect of storage and single layer centrifugation before cryopreservation on stallion sperm cryosurvival
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-04-26T12:11:06Z
dc.journal.volume
135
dc.journal.pagination
1-5
dc.journal.pais
Estados Unidos
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dc.description.fil
Fil: Nunes, M. M.. Universidade Federal de Viçosa.; Brasil
dc.description.fil
Fil: Morrell, J. M.. Swedish University Of Agricultural Sciences; Suecia
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Fil: Santos, F. C. C.. Universidade Federal dos Vales Jequitinhonha e Mucuri; Brasil
dc.description.fil
Fil: Miragaya, M. H.. Universidad de Buenos Aires; Argentina
dc.description.fil
Fil: Gallelli, Maria Florencia. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Area de Teriogenología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Rodriguez, Marcelo. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina
dc.description.fil
Fil: Guimaraes, J. D.. Universidade Federal de Viçosa.; Brasil
dc.journal.title
Journal of Equine Veterinary Science
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.jevs.2024.105046
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