Artículo
Dehydroleucodine and xanthatin, two natural anti-inflammatory lactones, inhibit mast cell degranulation by affecting the actin cytoskeleton
Wetten, Paula
; Arismendi Sosa, Andrea Celeste
; Mariani, María Laura Lucía; Vargas, Patricia Mercedes; Michaut, Marcela Alejandra
; Penissi, Alicia Beatriz
Fecha de publicación:
10/2023
Editorial:
John Wiley and Sons Inc
Revista:
Cytoskeleton
ISSN:
1949-3584
e-ISSN:
1949-3592
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Actin remodeling is a critical regulator of mast cell secretion. In previous work, we have shown that dehydroleucodine and xanthatin, two natural α,β-unsaturated lactones, exhibit anti-inflammatory and mast cell stabilizing properties. Based on this background, this study aimed to determine whether the mast cell stabilizing action of these lactones is associated with changes in the actin cytoskeleton. Rat peritoneal mast cells were preincubated in the presence of dehydroleucodine or xanthatin before incubation with compound 48/80. Comparative studies with sodium cromoglycate and latrunculin B were also made. After treatments, different assays were performed on mast cell samples: β-hexosaminidase release, cell viability studies, quantification of mast cells and their state of degranulation by light microscopy, transmission electron microscopy, and actin staining for microscopy observation. Results showed that dehydroleucodine and xanthatin inhibited mast cell degranulation, evidenced by the inhibition of β-hexosaminidase release and decreased degranulated mast cell percentage. At the same time, both lactones altered the F-actin cytoskeleton in mast cells resulting, similarly to Latrunculin B, in a higher concentration of nuclear F-actin when activated by compound 48/80. For the first time, this study describes the biological properties of dehydroleucodine and xanthatin concerning to the rearrangement of actin filaments during stimulated exocytosis in mast cells. These data have important implications for developing new anti-inflammatory and mast cell stabilizing drugs and for designing new small molecules that may interact with the actin cytoskeleton.
Palabras clave:
ACTIN
,
DEHYDROLEUCODINE
,
LATRUNCULIN B
,
MAST CELLS
,
NUCLEAR F-ACTIN
,
XANTHATIN
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(IHEM)
Articulos de INST. HISTOLOGIA Y EMBRIOLOGIA DE MEND DR.M.BURGOS
Articulos de INST. HISTOLOGIA Y EMBRIOLOGIA DE MEND DR.M.BURGOS
Citación
Wetten, Paula; Arismendi Sosa, Andrea Celeste; Mariani, María Laura Lucía; Vargas, Patricia Mercedes; Michaut, Marcela Alejandra; et al.; Dehydroleucodine and xanthatin, two natural anti-inflammatory lactones, inhibit mast cell degranulation by affecting the actin cytoskeleton; John Wiley and Sons Inc; Cytoskeleton; 2023; 10-2023; 1-12
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