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dc.contributor.author
Peñaherrera Pazmiño, Ana Belén
dc.contributor.author
Payés, Cristian
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Sierra Rodero, Marina
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Vega, M.
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Rosero, G.
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Lerner, Betiana
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Helguera, Gustavo Fernando
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Pérez, M. S.
dc.date.available
2017-08-30T20:37:50Z
dc.date.issued
2016-03-31
dc.identifier.citation
Peñaherrera Pazmiño, Ana Belén; Payés, Cristian; Sierra Rodero, Marina; Vega, M.; Rosero, G.; et al.; Evaluation of cell culture in microfluidic chips for application in monoclonal antibody production; Elsevier Science; Microelectronic Engineering; 158; 31-3-2016; 126-129
dc.identifier.issn
0167-9317
dc.identifier.uri
http://hdl.handle.net/11336/23347
dc.description.abstract
Microfluidic chips are useful devices for cell culture that allow cell growth under highly controlled conditions, as is required for production of therapeutic recombinant proteins. To understand the optimal conditions for growth of cells amenable of recombinant protein expression in these devices,we culturedHEK-293T cells under different microfluidic experimental conditions. The cells were cultured in polymethyl methacrylate (PMMA) and polydi-methylsiloxane (PDMS)microdevices, in the absence or presence of the cell adhesion agent poly-D-lysine. Different microchannel geometries and thicknesses, as well as the influence of the flow rate have also been tested, showing their great influence in cell adhesion and growth. Results show that the presence of poly-D-lysine improves the adhesion and viability of the cells in continuous or discontinuous flow. Moreover, the optimal adhesion of cells was observed in the corners of themicrochannels, as well as in wide channels possibly due to the decrease in the flow rate in these areas. These studies provide insight into the optimal architecture of microchannels for long-term culture of adherent cells in order to use microfluidics devices as bioreactors for monoclonal antibodies production.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/embargoedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Microfluidics
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Microchannel
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Lab on A Chip
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Cell Culture
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Hek-293t Cells
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Bioprocesamiento Tecnológico, Biocatálisis, Fermentación
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Biotecnología Industrial
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INGENIERÍAS Y TECNOLOGÍAS
dc.title
Evaluation of cell culture in microfluidic chips for application in monoclonal antibody production
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-08-24T15:32:31Z
dc.journal.volume
158
dc.journal.pagination
126-129
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Peñaherrera Pazmiño, Ana Belén. Universidad Tecnológica Nacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Payés, Cristian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil
Fil: Sierra Rodero, Marina. Universidad Tecnológica Nacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Vega, M.. Universidad Tecnológica Nacional; Argentina
dc.description.fil
Fil: Rosero, G.. Universidad Tecnológica Nacional; Argentina. Universidad de Buenos Aires; Argentina
dc.description.fil
Fil: Lerner, Betiana. Universidad Tecnológica Nacional; Argentina. Universidad de Buenos Aires; Argentina
dc.description.fil
Fil: Helguera, Gustavo Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil
Fil: Pérez, M. S.. Universidad Tecnológica Nacional; Argentina. Universidad de Buenos Aires; Argentina
dc.journal.title
Microelectronic Engineering
dc.rights.embargoDate
2018-04-30
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0167931716301861
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.mee.2016.03.059
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