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dc.date.available
2024-04-18T14:31:18Z
dc.identifier.citation
Schröder, Noelia Malena; Chelaliche, Anibal Sebastian; Rodríguez, María Daniela; Zapata, Pedro Dario; Fonseca, Maria Isabel; (2024): Phlebia brevispora BAFC633 proteome. Consejo Nacional de Investigaciones Científicas y Técnicas. (dataset). http://hdl.handle.net/11336/233474
dc.identifier.uri
http://hdl.handle.net/11336/233474
dc.description.abstract
This is the proteome (NS01 and NS02) and secretome (NS03 and NS04) of Phlebia brevispora BAF633 obtained from the mycellium of the fungus harvested in a liquid malt extract medium supplemented with L-tyrosine. The data was obtained via nanoHPCL MS/MS and by using the proteome discover software. This dataset contains the accesion number of every protein identified.
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.title
Phlebia brevispora BAFC633 proteome
dc.type
dataset
dc.date.updated
2024-04-18T14:09:20Z
dc.description.fil
Fil: Schröder, Noelia Malena. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Bioquímica Clinica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
dc.description.fil
Fil: Chelaliche, Anibal Sebastian. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Bioquímica Clinica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
dc.description.fil
Fil: Rodríguez, María Daniela. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Bioquímica Clinica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
dc.description.fil
Fil: Zapata, Pedro Dario. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Bioquímica Clinica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
dc.description.fil
Fil: Fonseca, Maria Isabel. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Bioquímica Clinica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
dc.datacite.PublicationYear
2024
dc.datacite.Creator
Schröder, Noelia Malena
dc.datacite.Creator
Chelaliche, Anibal Sebastian
dc.datacite.Creator
Rodríguez, María Daniela
dc.datacite.Creator
Zapata, Pedro Dario
dc.datacite.Creator
Fonseca, Maria Isabel
dc.datacite.affiliation
Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Bioquímica Clinica
dc.datacite.affiliation
Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste
dc.datacite.affiliation
Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Bioquímica Clinica
dc.datacite.affiliation
Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste
dc.datacite.affiliation
Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Bioquímica Clinica
dc.datacite.affiliation
Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste
dc.datacite.affiliation
Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Bioquímica Clinica
dc.datacite.affiliation
Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste
dc.datacite.affiliation
Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Bioquímica Clinica
dc.datacite.affiliation
Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste
dc.datacite.publisher
Consejo Nacional de Investigaciones Científicas y Técnicas
dc.datacite.subject
Bioproductos, Biomateriales, Bioplásticos, Biocombustibles, Bioderivados, etc.
dc.datacite.subject
Biotecnología Industrial
dc.datacite.subject
INGENIERÍAS Y TECNOLOGÍAS
dc.datacite.date
27/02/2024
dc.datacite.DateType
Creado
dc.datacite.language
eng
dc.datacite.version
1.0
dc.datacite.description
For the whole proteome analysis, the 8 days mycelium culture of P. brevispora was first subjected to a cellular breakdown using liquid nitrogen. After this, protein extraction was performed: 500 mL of extraction buffer was added to the lysate, incubated for 1h at room temperature and then centrifuged to obtain the protein extract. For the secretome analysis, the supernatant was clarified to remove remaining cells using 0.22 mm filters (Chromafil®xtra). The protein concentration of each sample was determined using the Pierce™ BCA protein Assay Kit (Thermo Scientific). Each sample was then reduced with 10 mM dithiothreitol (DTT) at 56°C for 45 minutes and alkylated with a solution of 20 mM iodoacetamide at room temperature for 45 minutes in darkness. Finally, samples were precipitated with trichloroacetic acid for 2 h at 20°C and washed three times with cold acetone (4 °C). The obtained pellets were analyzed by a nanoHPCL EASY-nLC 1000 at the Center for Chemical and Biological Studies by Mass Spectrometry, University of Buenos Aires, Argentina (http://cequibiem.qb.fcen.uba.ar). The obtained pellets of both procedures were suspended in ammonium bicarbonate buffer (50 mM, pH 8) and digested with trypsin overnight. Samples then were freeze dried using SpeedVac and resuspended in 30ul of trifluoroacetic acid 0.1%. A final desalting process was carried out using a C18 Zip Tip (Merck). The samples were passed through an EASY-Spray Accucore (P/N ES801) column (C18, 2um, 100A, 75 um x 150 mm) using a temperature of 35°C, a flow of 300 nL/min and a gradient of water with 0.1% of formic acid (solution “A”) and acetonitrile with 0.1% of formic acid (solution “B”). Peptides were separated by using a gradient of 30% of B solution for 60 min and a gradient of 95% of B solution for 15 minutes. The mass spectra were obtained using a Q-exactive spectrometer (Thermo Scientific). The 12 most intense signal obtained in the Full-scan MS were selected for MS/MS spectra. All of these spectra were analyzed by Proteome Discoverer using Phlebia, Agaricus, Trametes and Pleurotus data bases, with 2 miscleavages allowed, a precursor ion mass tolerance of 10 ppm, a fragmented ion mass tolerance of 0.05Da and using Oxidation as a dynamic modification and Carbamidomethylation as a static modification.
dc.datacite.DescriptionType
Métodos
dc.subject.keyword
Mycology
dc.subject.keyword
Proteome
dc.subject.keyword
Enzyme
dc.subject.keyword
Secretion
dc.datacite.resourceTypeGeneral
dataset
dc.conicet.datoinvestigacionid
14948
dc.datacite.geolocation
Misiones
dc.datacite.formatedDate
2024
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