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dc.contributor.author
Bianchi, M.
dc.contributor.author
Paravani, Enrique Valentin
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dc.contributor.author
Acosta, M. G.
dc.contributor.author
Odetti, Lucia Magdalena
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dc.contributor.author
Simoniello, Maria Fernanda
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dc.contributor.author
Poletta, Gisela Laura
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dc.date.available
2024-04-18T13:22:09Z
dc.date.issued
2024-06
dc.identifier.citation
Bianchi, M.; Paravani, Enrique Valentin; Acosta, M. G.; Odetti, Lucia Magdalena; Simoniello, Maria Fernanda; et al.; Pesticide-induced alterations in zebrafish (Danio rerio) behavior, histology, DNA damage and mRNA expression: An integrated approach; Elsevier Science Inc.; Comparative Biochemistry And Physiology. Toxicology & Pharmacology; 280; 6-2024; 1-10
dc.identifier.issn
1532-0456
dc.identifier.uri
http://hdl.handle.net/11336/233452
dc.description.abstract
To assess the impact of glyphosate and 2,4-D herbicides, as well as the insecticide imidacloprid, both individually and in combination, the gills of adult zebrafish were used due to their intimate interaction with chemicals diluted in water. Bioassays were performed exposing the animals to the different pesticides and their mixture for 96 h.The behavior of the fish was analyzed, a histological examination of the gills was carried out, and the genotoxic effects were also analyzed by means of the comet assay (CA) and the change in the expression profiles of genes involved in the pathways of the oxidative stress and cellular apoptosis. The length traveled and the average speed of the control fish, compared to those exposed to the pesticides and mainly those exposed to the mixture, were significantly greater. All the groups exposed individually exhibited a decrease in thigmotaxis time, indicating a reduction in the behavior of protecting themselves from predators. Histological analysis revealed significant differences in the structures of the gill tissues. The quantification of the histological lesions showed mild lesionsin the fish exposed to imidacloprid, moderate to severe lesions for glyphosate, and severe lesions in the case of 2,4-D and the mixture of pesticides. The CA revealed the sensitivity of gill cells to DNA damage following exposure to glyphosate, 2,4-D, imidacloprid and the mixture. Finally, both genes involved in the oxidative stress pathway and those related to the cell apoptosis pathway were overexpressed, while the ogg1 gene, involved in DNA repair, was downregulated.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science Inc.
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dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Glyphosate
dc.subject
2,4-D
dc.subject
Imidacloprid
dc.subject
Zebrafish gills
dc.subject
Glyphosate
dc.subject
Imidacloprid
dc.subject.classification
Otras Ciencias Biológicas
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Pesticide-induced alterations in zebrafish (Danio rerio) behavior, histology, DNA damage and mRNA expression: An integrated approach
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-04-10T12:00:57Z
dc.identifier.eissn
1878-1659
dc.journal.volume
280
dc.journal.pagination
1-10
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Bianchi, M.. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina
dc.description.fil
Fil: Paravani, Enrique Valentin. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina
dc.description.fil
Fil: Acosta, M. G.. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina
dc.description.fil
Fil: Odetti, Lucia Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Cátedra de Toxicología y Bioquímica Legal; Argentina
dc.description.fil
Fil: Simoniello, Maria Fernanda. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Cátedra de Toxicología y Bioquímica Legal; Argentina
dc.description.fil
Fil: Poletta, Gisela Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Cátedra de Toxicología y Bioquímica Legal; Argentina
dc.journal.title
Comparative Biochemistry And Physiology. Toxicology & Pharmacology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S1532045624000632
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.cbpc.2024.109895
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