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dc.contributor.author
Potikha, Tamara
dc.contributor.author
Ella, Ezra
dc.contributor.author
Cerliani, Juan Pablo
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dc.contributor.author
Mizrahi, Lina
dc.contributor.author
Pappo, Orit
dc.contributor.author
Rabinovich, Gabriel Adrián
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dc.contributor.author
Galun, Eithan
dc.contributor.author
Goldenberg, Daniel S.
dc.date.available
2017-08-30T20:14:24Z
dc.date.issued
2016-05-05
dc.identifier.citation
Potikha, Tamara; Ella, Ezra; Cerliani, Juan Pablo; Mizrahi, Lina; Pappo, Orit; et al.; Galectin-1 is essential for efficient liver regeneration following hepatectomy; Impact journals; Oncotarget; 7; 22; 5-5-2016; 31738-31754
dc.identifier.issn
1949-2553
dc.identifier.uri
http://hdl.handle.net/11336/23338
dc.description.abstract
Galectin-1 (Gal1) is a known immune/inflammatory regulator which actsboth extracellularly and intracellularly, modulating innate and adaptive immuneresponses. Here, we explored the role of Gal1 in liver regeneration using 70% partial hepatectomy (PHx) of C57BL/6 wild type and Gal1-knockout (Gal1-KO, Lgals1-/-) mice. Gene or protein expression, in liver samples collected at time intervals from 2 to 168 hours post-operation, was tested by either RT-PCR or by immunoblotting and immunohistochemistry, respectively. We demonstrated that Gal1 transcript and protein expression was induced in the liver tissue of wild type mice upon PHx. Liver regeneration following PHx was significantly delayed in the Gal1-KO compared to the control liver. This delay was accompanied by a decreased Akt phosphorylation, and accumulation of the hepatocyte nuclear p21 protein in the Gal1-KO versus control livers at 24 and 48 hours following PHx. Transcripts of several known regulators of inflammation, cell cycle and cell signaling, including some known PHx-induced genes, were aberrantly expressed (mainly down-regulated) in Gal1-KO compared to control livers at 2, 6 and 24 hours post-PHx. Transient steatosis, which is imperative for liver regeneration following PHx, was significantly delayed and decreased in the Gal1- KO compared to the control liver and was accompanied by a significantly decreased expression in the mutant liver of several genes encoding lipid metabolism regulators.Our results demonstrate that Gal1 protein is essential for efficient liver regeneration following PHx through the regulation of liver inflammation, hepatic cell proliferation, and the control of lipid storage in the regenerating liver.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Impact journals
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Liver Regeneration
dc.subject
Galectin-1
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Hepatectomy
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Lipid Metabolism
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Medicina Básica
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Galectin-1 is essential for efficient liver regeneration following hepatectomy
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-08-24T15:32:21Z
dc.identifier.eissn
1949-2553
dc.journal.volume
7
dc.journal.number
22
dc.journal.pagination
31738-31754
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Albania
dc.description.fil
Fil: Potikha, Tamara. Hadassah Hebrew University Medical Center; Israel
dc.description.fil
Fil: Ella, Ezra. Hadassah Hebrew University Medical Center; Israel
dc.description.fil
Fil: Cerliani, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil
Fil: Mizrahi, Lina. Hadassah Hebrew University Medical Center; Israel
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Fil: Pappo, Orit. Hadassah Hebrew University Medical Center; Israel
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Fil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
dc.description.fil
Fil: Galun, Eithan. Hadassah Hebrew University Medical Center; Israel
dc.description.fil
Fil: Goldenberg, Daniel S.. Hadassah Hebrew University Medical Center; Israel
dc.journal.title
Oncotarget
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path[]=9194&pubmed-linkout=1
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.18632/oncotarget.9194
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5077973/
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