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dc.contributor.author
Saravi, Fernando Daniel
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dc.contributor.author
Carra, Graciela Ester
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dc.contributor.author
Matus, Daniel A.
dc.contributor.author
Ibañez, Jorge Ernesto
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dc.date.available
2024-04-10T12:55:57Z
dc.date.issued
2017-03
dc.identifier.citation
Saravi, Fernando Daniel; Carra, Graciela Ester; Matus, Daniel A.; Ibañez, Jorge Ernesto; Rectification of oxygen transfer through the rat colonic epithelium; Baishideng Publishing Group; World Journal of Gastrointestinal Pathophysiology; 8; 2; 3-2017; 59-66
dc.identifier.issn
2150-5330
dc.identifier.uri
http://hdl.handle.net/11336/232620
dc.description.abstract
AIMTo assess whether higher sensitivity of colonic epithelium to hypoxia at the serosal side is associated with oxygen transfer asymmetry.METHODSRats were fed either with normal chow or a low-sodium diet. Tissues were mounted as flat sheets in a modified, airtight Ussing chamber with oxygen meters in each hemichamber. Mucosal samples from normal diet animals were studied under control conditions, in low-chloride solution and after adding chloride secretion inhibitors and chloride secretagogues. Samples from sodium-deprived rats were studied before and after ouabain addition. In separate experiments, the correlation between short-circuit current and oxygen consumption was analyzed. Finally, hypoxia was induced in one hemichamber to assess the relationship between its oxygen content and the oxygen pressure difference between both hemichambers.RESULTSIn all studied conditions, oxygen consumption was larger in the serosal hemichamber than in the mucosal one (P = 0.0025 to P < 0.0001). Short-circuit current showed significant correlation with both total oxygen consumption (r = 0.765; P = 0.009) in normoxia and oxygen consumption in the serosal hemichamber (r = 0.754; P = 0.011) during mucosal hypoxia, but not with oxygen consumption in the mucosal hemichamber. When hypoxia was induced in the mucosal hemichamber, an oxygen pressure difference of 13 kPa with the serosal hemichamber was enough to keep its oxygen content constant. However, when hypoxia was induced in the serosal hemichamber, the oxygen pressure difference with the mucosal hemichamber necessary to keep its oxygen content constant was 40 kPa (P < 0.0001).CONCLUSIONSerosal oxygen supply is more readily available to support short-circuit current. This may be partly due to a rectifying behavior of transepithelial oxygen transfer.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Baishideng Publishing Group
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.subject
COLONIC EPITHELIUM
dc.subject
SHORT-CIRCUIT CURRENT, SHORT-CIRCUIT CURRENT
dc.subject
HYPOXIA
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USSING CHAMBER
dc.subject
OXYGEN DIFFUSION
dc.subject.classification
Otras Medicina Básica
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dc.subject.classification
Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
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Biofísica
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
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Otras Ciencias de la Salud
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dc.subject.classification
Ciencias de la Salud
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CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Rectification of oxygen transfer through the rat colonic epithelium
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-04-03T13:37:59Z
dc.journal.volume
8
dc.journal.number
2
dc.journal.pagination
59-66
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
California
dc.description.fil
Fil: Saravi, Fernando Daniel. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Fisiología; Argentina
dc.description.fil
Fil: Carra, Graciela Ester. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Fisiología; Argentina
dc.description.fil
Fil: Matus, Daniel A.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
dc.description.fil
Fil: Ibañez, Jorge Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
dc.journal.title
World Journal of Gastrointestinal Pathophysiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.wjgnet.com/2150-5330/full/v8/i2/59.htm
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.4291/wjgp.v8.i2.59
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