Capítulo de Libro
Development, Production, and Characterization of Hepatitis B Subviral Envelope Particles as a Third-Generation Vaccine
Título del libro: Vaccine Design: Methods and Protocols. Vaccines for Human Diseases
Battagliotti, Juan Manuel
; Fontana, Diego Sebastian
; Etcheverrigaray, Marina
; Kratje, Ricardo Bertoldo
; Prieto, Claudio
Fecha de publicación:
2022
Editorial:
Springer
ISBN:
978-1-0716-1884-4
Idioma:
Inglés
Clasificación temática:
Resumen
Vaccination still represents the most efficient and inexpensive strategy in the control of hepatitis B virus (HBV) infection. However, about 10% of the population vaccinated with the current S yeast-derived vaccine fail to induce an adequate immune response. Our group has developed a new-generation hepatitis B vaccine candidate composed by the three surface proteins of the HBV. Here we describe the methods to develop and characterize a stable CHO-K1 recombinant cell line able to produce and secrete hepatitis B subviral envelope particles (HBV-SVPs) containing L and M glycoproteins in addition to S glycoprotein. In addition, Western blot and immunogold electron microscopy techniques to evaluate the size, morphology, and composition of the particles are explained. Finally, immunization protocols are described in order to study the immunogenicity of HBV-SVPs and the ability of the antibodies triggered by these particles to recognize the binding site of HBV with the hepatocyte.
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Capítulos de libros(CCT - SANTA FE)
Capítulos de libros de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Capítulos de libros de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Citación
Battagliotti, Juan Manuel; Fontana, Diego Sebastian; Etcheverrigaray, Marina; Kratje, Ricardo Bertoldo; Prieto, Claudio; Development, Production, and Characterization of Hepatitis B Subviral Envelope Particles as a Third-Generation Vaccine; Springer; 2022; 273-287
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