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Artículo

A simple method for the assessment of electrophoretic mobility in porous media

Franck, NicolasIcon ; Vera Candioti, LucianaIcon ; Gerlero, Gabriel SantiagoIcon ; Urteaga, RaulIcon ; Kler, Pablo AlejandroIcon
Fecha de publicación: 10/2023
Editorial: Wiley VCH Verlag
Revista: Electrophoresis
ISSN: 0173-0835
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Físico-Química, Ciencia de los Polímeros, Electroquímica

Resumen

Developing paper-based electrophoretic methods involve dealing with significant uncertainty levels when compared to their capillary counterparts. Critical information for developing these kinds of methods are the electrophoretic mobility of background electrolytes and samples. This work presents the design and characterization of a device for measuring the electrophoretic mobilities of dyes in porous media. The device was developed with the aim of validating a previously presented model and also proposing a protocol for the straightforward determination of electrophoretic mobilities in porous media when open-channel values are already known. Whatman #1 paper was used as a model substrate as far as it is the most common porous medium substrate for paper-based electrophoresis. The device was designed using a numerical simulation–assisted approach, utilizing OpenFOAM® and specific solvers for capillary transport and electromigration, namely porousMicroTransport and electroMicroTransport, respectively. The electrophoretic mobilities of five dyes were analyzed experimentally with the proposed device. To establish appropriate comparative values at different pHs, experiments in fused silica capillaries were also performed. An effective parameter model for describing the electrophoretic behavior of dyes in porous media, that is, the constriction factor, was found consistent with previous reports for the Whatman #1 paper. This consistency was found after considering (via direct measurements) the chromatographic effect of the medium over each dye. Consequently, the recorded values hold significant worth due to their potential for direct application in designing new experiments or devices in Whatman #1 paper. With the validation of the model through the experiments with the proposed device, those researchers interested on developing electrophoretic methods in porous substrates can make use of the open-channel electrophoretic mobilities reported in the literature, or in the well-known software databases, and correct them for the media of interest just by performing two simple characterization steps.
Palabras clave: DYES , E-ΜPADS , ELECTROPHORESIS , NUMERICAL SIMULATIONS , PAPER-BASED MICROFLUIDICS
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/232302
DOI: http://dx.doi.org/10.1002/elps.202300180
URL: https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/elps.20230
Colecciones
Articulos(CCT - SANTA FE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Articulos(CIMEC)
Articulos de CENTRO DE INVESTIGACION DE METODOS COMPUTACIONALES
Articulos(IFIS - LITORAL)
Articulos de INST.DE FISICA DEL LITORAL
Citación
Franck, Nicolas; Vera Candioti, Luciana; Gerlero, Gabriel Santiago; Urteaga, Raul; Kler, Pablo Alejandro; A simple method for the assessment of electrophoretic mobility in porous media; Wiley VCH Verlag; Electrophoresis; 10-2023; 1-10
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