Artículo
Advancing ocular safety research: A comprehensive examination of benzocaine acute exposure without animal testing
Lenze, Mariela Belén
; Benedetti, Martina Daniela
; Roco, Julieta
; Ramírez, Pedro Germán
; Blanco, Raúl; Yaceszen, Sol; Corrales, Carlos; Wikinski, Silvia Ines
; Gutiérrez, María Laura
Fecha de publicación:
03/2024
Editorial:
Elsevier Ireland
Revista:
Toxicology Letters
ISSN:
0378-4274
e-ISSN:
1879-3169
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Benzocaine is a widely employed local anaesthetic; however, there is a notable dearth of preclinical and clinical evidence regarding its safety in ophthalmological products. To address this, a comprehensive strategy incorporating in silico and in vitro methodologies was proposed for assessing benzocaine´s ocular toxicity without animal testing. To collect the in silico evidence, the QSAR Toolbox (v4.5) was used. A single exposure to two benzocaine concentrations (2% and 20%) was evaluated by in vitro methods. Hen’s Egg Chorioallantoic Membrane Test (HET-CAM) was performed to evaluate the effects on the conjunctiva. To study corneal integrity, Short Time Exposure test (STE) and Bovine Corneal Opacity and Permeability (BCOP) assay, followed by histopathological analysis, were carried out. Results from both in silico and in vitro methodologies categorize benzocaine as non-irritating. The histopathological analysis further affirms the safety of using benzocaine in eye drops, as no alterations were observed in evaluated corneal strata. This research proposes a useful combined strategy to provide evidence on the safety of local anaesthetics and particularly show that 2% and 20% benzocaine solutions do not induce eye irritation or corneal damage, supporting the potential use of benzocaine in the development of ophthalmic anesthetic products.
Palabras clave:
Benzocaine
,
Ocular
,
Safety
,
Toxicology
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(OCA HOUSSAY)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Citación
Lenze, Mariela Belén; Benedetti, Martina Daniela; Roco, Julieta; Ramírez, Pedro Germán; Blanco, Raúl; et al.; Advancing ocular safety research: A comprehensive examination of benzocaine acute exposure without animal testing; Elsevier Ireland; Toxicology Letters; 394; 3-2024; 138-145
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