Evento
Cholesterol modulates the rate and mechanism of acetylcholine receptor internalization
Tipo del evento:
Congreso
Nombre del evento:
XXVI Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias
Fecha del evento:
18/10/2011
Institución Organizadora:
Sociedad Argentina de Investigación en Neurociencias;
Título del Libro:
XXVI Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias
Editorial:
Sociedad Argentina de Investigación en Neurociencias
Idioma:
Español
Clasificación temática:
Resumen
Stability of the nicotinic acetylcholine receptor (AChR) at the cell surface is key to the correct functioning of the cholinergic synapse. Cholesterol (Chol) is necessary for homeostasis of AChR levels at the plasmalemma and for ion translocation. Here we characterize the endocytic pathway followed by muscle-type AChR in Chol-depleted cells (Chol(-)). Under such conditions, the AChR is internalized by a ligand-, clathrin-, and dynamin-independent mechanism. Expression of a dominant negative form of the small GTPase Rac1, Rac1N17, abolishes receptor endocytosis. Unlike the endocytic pathway in control CHO cells, accelerated AChR internalization proceeds even upon disruption of the actin cytoskeleton. Under Chol(-) conditions, AChR internalization is furthermore found to require the activity of Arf6 and its effectors Rac1 and phospholipase D. The Arf6-dependent mechanism may constitute the default endocytic pathway followed by the AChR in the absence of external ligands, membrane Chol levels acting as a key homeostatic regulator of cell surface receptor levels
Palabras clave:
ACHR
,
CHOLESTEROL
,
ENDOCYTOSIS
,
ARF6
Archivos asociados
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Identificadores
Colecciones
Eventos(INIBIBB)
Eventos de INST.DE INVEST.BIOQUIMICAS BAHIA BLANCA (I)
Eventos de INST.DE INVEST.BIOQUIMICAS BAHIA BLANCA (I)
Citación
Cholesterol modulates the rate and mechanism of acetylcholine receptor internalization; XXVI Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias; Huerta Grande, Cordoba; Argentina; 2011; 76-76
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