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dc.contributor.author
Chrestia, Juan Facundo  
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Bouzat, Cecilia Beatriz  
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Esandi, María del Carmen  
dc.date.available
2024-04-05T16:02:10Z  
dc.date.issued
2018  
dc.identifier.citation
Phosphorylation of intracellular tyrosines modulates the ionotropic function of the α7 nicotinic receptor; XXXIII Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias; Córdoba; Argentina; 2018; 312-312  
dc.identifier.uri
http://hdl.handle.net/11336/232189  
dc.description.abstract
α7 is expressed in the brain and contributes to cognition, attention, and memory. It contains an extracellular domain with the agonist binding sites; a transmembrane domain, which forms the ion pore; and an intracellular domain (ICD), which contains sites for modulation and intracellular signaling. The mechanisms by which the cell can regulate the ionotropic function of α7 remain unknown. We explored how intracellular phosphorylation affects α7 activity by patch clamp recordings in HEK cells expressing α7. Wild-type α7 channel activity elicited by ACh appears as brief isolated openings and as activation episodes containing a few brief openings in quick succession (bursts). Preincubation of cells expressing α7 with the inhibitor of Src family kinases (PP2) increased significantly the mean burst duration. The exposure of cells to PP2 during the course of the recording revealed a significant increase in the frequency of channel opening in addition to the increase of burst durations. To confirm that these changes were due to the inhibition of phosphorylation of α7-ICD, we introduced mutations at potential phosphorylation sites (Y386F and Y442F). The mutations prolonged burst durations, thus mimicking the effects of PP2. Also, the mutants were insensitive to PP2, confirming that Y386 and Y442 are responsible for its effects on α7 kinetics. Our results indicate that dephosphorylation positively modulates α7 channel activity in a way compatible with decreased desensitization.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Sociedad Argentina de Investigación en Neurociencias  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
NICOTINIC RECEPTOR  
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TYROSYNES  
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Bioquímica y Biología Molecular  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Phosphorylation of intracellular tyrosines modulates the ionotropic function of the α7 nicotinic receptor  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.type
info:eu-repo/semantics/conferenceObject  
dc.type
info:ar-repo/semantics/documento de conferencia  
dc.date.updated
2024-03-25T15:41:10Z  
dc.journal.pagination
312-312  
dc.journal.pais
Argentina  
dc.journal.ciudad
Córdoba  
dc.description.fil
Fil: Chrestia, Juan Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina  
dc.description.fil
Fil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina  
dc.description.fil
Fil: Esandi, María del Carmen. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://saneurociencias.org.ar/congresos-san-2/  
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Autor  
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Autor  
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Autor  
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Nacional  
dc.type.subtype
Congreso  
dc.description.nombreEvento
XXXIII Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias  
dc.date.evento
2018-10-24  
dc.description.ciudadEvento
Córdoba  
dc.description.paisEvento
Argentina  
dc.type.publicacion
Book  
dc.description.institucionOrganizadora
Sociedad Argentina de Investigación en Neurociencias  
dc.source.libro
XXXIII Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias  
dc.date.eventoHasta
2018-10-26  
dc.type
Congreso