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dc.contributor.author
Sguazza, Guillermo Hernán
dc.contributor.author
Reynaldi, Francisco José
dc.contributor.author
Galosi, Cecilia Monica
dc.contributor.author
Pecoraro, Marcelo Ricardo
dc.date.available
2017-08-28T22:40:18Z
dc.date.issued
2013-08
dc.identifier.citation
Sguazza, Guillermo Hernán; Reynaldi, Francisco José; Galosi, Cecilia Monica; Pecoraro, Marcelo Ricardo; Simultaneous detection of bee viruses by multiplex PCR
; Elsevier Science; Journal of Virological Methods; 194; 1-2; 8-2013; 102-106
dc.identifier.issn
0166-0934
dc.identifier.uri
http://hdl.handle.net/11336/23204
dc.description.abstract
Honey bee mortality is a serious problem that beekeepers in Argentina have had to face during the last 3 years. Itis known thatthe consequence ofthe complex interactions between environmental and beekeeping parameters added to the effect of different disease agents such as viruses, bacteria, fungi and parasitic mites may result in a sudden collapse of the colony. In addition, multiple viral infections are detected frequently concomitantly in bee colonies. The aim of this study was to establish a multiplex polymerase chain reaction method for rapid and simultaneous detection of the most prevalent bee viruses. This multiplex PCR assay will provide specific, rapid and reliable results and allow for the cost effective detection of a particular virus as well as multiple virus infections in a single reaction tube. This method could be a helpful tool in the surveillance of the most frequently found bee viruses and to study the dynamics and the interactions of the virus populations within colonies.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Apis Mellifera
dc.subject
Bee Viruses
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Multiplex Rt-Pcr
dc.subject
Detection
dc.subject.classification
Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Simultaneous detection of bee viruses by multiplex PCR
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-08-28T14:31:05Z
dc.journal.volume
194
dc.journal.number
1-2
dc.journal.pagination
102-106
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Sguazza, Guillermo Hernán. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Reynaldi, Francisco José. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Galosi, Cecilia Monica. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
dc.description.fil
Fil: Pecoraro, Marcelo Ricardo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Journal of Virological Methods
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0166093413003352
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.jviromet.2013.08.003
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