Mostrar el registro sencillo del ítem
dc.contributor.author
Vanagas, Laura
dc.contributor.author
Dalmasso, Maria Carolina
dc.contributor.author
Dubremetz, Jean F.
dc.contributor.author
Portiansky, Enrique Leo
dc.contributor.author
Olins, Donald E.
dc.contributor.author
Angel, Sergio Oscar
dc.date.available
2017-08-28T21:57:22Z
dc.date.issued
2013-05
dc.identifier.citation
Vanagas, Laura; Dalmasso, Maria Carolina; Dubremetz, Jean F.; Portiansky, Enrique Leo; Olins, Donald E.; et al.; Epichromatin is conserved in Toxoplasma gondii and labels the exterior parasite chromatin throughout the cell cycle; Cambridge University Press; Parasitology; 140; 9; 5-2013; 1104-1110
dc.identifier.issn
0031-1820
dc.identifier.uri
http://hdl.handle.net/11336/23200
dc.description.abstract
Toxoplasma gondii is an Apicomplexan intracellular protozoan parasite responsible for Toxoplasmosis, a disease with considerable medical and economic impact worldwide. T. gondii cells never lose the nuclear envelope and their chromosomes do not present a higher order level of condensation during mitosis. Here, we tested the murine monoclonal antibody PL2-6, which labels epichromatin, a conformational chromatin epitope based on DNA+H2As and H2Bs, in T. gondii cultured on fibroblast cells. This epitope is present on the exterior chromatin surface of interphase nuclei and on the periphery of metaphasic chromosomes in higher eukaryotes. PL2-6 reacted with T. gondii H2As and H2Bs histones in Western blot assays. Besides, the antibody reacted with the nuclear fraction of tachyzoites, as a single band coincident with H2B histone. In the T. gondii tachyzoite stage, PL2-6 also had peripheral nuclear localization, as observed by epifluorescence/confocal microscopy and also immunoelectron microscopy. Confocal analysis showed that epichromatin is slightly polarized to one face of the parasite exterior chromatin surface. In replicating tachyzoites, PL2-6 also labels the exterior chromatin surface, covering the face of both segregating nuclei, facing the plasma membrane of the mother cell. The possible role of epichromatin in T. gondii is discussed.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Cambridge University Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Toxoplasma Gondii
dc.subject
Epichromatin
dc.subject
Nuclear Envelope
dc.subject
Cell Cycle
dc.subject
Replication
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Epichromatin is conserved in Toxoplasma gondii and labels the exterior parasite chromatin throughout the cell cycle
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-08-14T19:50:30Z
dc.journal.volume
140
dc.journal.number
9
dc.journal.pagination
1104-1110
dc.journal.pais
Reino Unido
dc.journal.ciudad
Cambridge
dc.description.fil
Fil: Vanagas, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
dc.description.fil
Fil: Dalmasso, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
dc.description.fil
Fil: Dubremetz, Jean F.. Universite Montpellier II; Francia
dc.description.fil
Fil: Portiansky, Enrique Leo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Olins, Donald E.. University of New England; Estados Unidos
dc.description.fil
Fil: Angel, Sergio Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
dc.journal.title
Parasitology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1017/S0031182013000504
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/parasitology/article/epichromatin-is-conserved-in-toxoplasma-gondii-and-labels-the-exterior-parasite-chromatin-throughout-the-cell-cycle/18CD42DA377C002BA1D8C81545577C3D
Archivos asociados