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Evento

Changes in biophysical properties of membranes containing sphingomyelin with very long chain PUFA induced by its hydrolysis

Peñalva, Daniel AlejandroIcon ; Fanani, M.L.; Maggio, BrunoIcon ; Aveldaño, Marta IsabelIcon ; Antollini, Silvia SusanaIcon
Tipo del evento: Congreso
Nombre del evento: XLII Reunión Anual de la Sociedad Argentina de Biofísica
Fecha del evento: 02/12/2013
Institución Organizadora: Sociedad Argentina de Biofísica;
Título del Libro: XLII Reunión Anual de la Sociedad Argentina de Biofísica
Editorial: Sociedad Argentina de Biofísica
ISBN: 978-987-27591-2-4
Idioma: Inglés
Clasificación temática:
Biofísica

Resumen

Very long-chain (C24 to C36) polyunsaturated fatty acids (VLCPUFA) are important acyl groups of sphingomyelin (SM) and ceramide (Cer) of mammalian spermatozoa. In rat sperm, SM species containing PUFA with 28-32 carbon atoms are exclusively located on the heads. After inducing the acrosomal reaction almost complete hydrolysis such SMs occurs, leading to gametes considerably enriched in the corresponding Cer species. The aim of this study was to evaluate the effects of the sphingomyelinaseinduced conversion VLCPUFA-SM → VLCPUFA-Cer on the biophysical properties of a binary model system (POPC/SM). The VLCPUFA-containing molecular species of SM were isolated from rat testes by a combination of chromatographic techniques. Egg-SM, whose properties are widely known, was used for comparison. Unilamellar liposomes (LUVs) were prepared and Dynamic Light Scattering was used to evaluate their structures/sizes before and after enzymatic hydrolysis. The potential increase in the interaction between different populations of liposomes (fission/fussion) after the SM → Cer hydrolysis was evaluated by FRET assays using NBD-PE as donor and Rh-PE as acceptor, and the lateral segregation of phases after Cer generation was followed by anisotropy of different fluorescence probes (laurdan, DPH and NBD-PE). In all biophysical properties measured, the SM species containing VLCPUFA contrasted with those of the egg-derived saturated SM. The longer and bulkier acyl chains of VLCPUFA-Cer may play a role in favouring the fusion/fission events that occur in the head of spermatozoa during the acrosomal reaction, a process that requires topological lipid intermediates with negative curvature
Palabras clave: SPHINGOMYELIN , CERAMIDE , BIOPHYSICAL PROPERTIES , FUSSION
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/231861
URL: https://biofisica.org.ar/reuniones-cientificas/reunionsab-previas/
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Eventos(INIBIBB)
Eventos de INST.DE INVEST.BIOQUIMICAS BAHIA BLANCA (I)
Citación
Changes in biophysical properties of membranes containing sphingomyelin with very long chain PUFA induced by its hydrolysis; XLII Reunión Anual de la Sociedad Argentina de Biofísica; Córdoba; Argentina; 2013; 40-40
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