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dc.contributor.author
López Fontana, Constanza Matilde
dc.contributor.author
Sasso, Corina Verónica
dc.contributor.author
Maselli, María Eugenia
dc.contributor.author
Santiano, Flavia Eliana
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Semino, Silvana Noemi
dc.contributor.author
Cuello Carrión, Fernando Darío
dc.contributor.author
Jahn, Graciela Alma
dc.contributor.author
Caron, Ruben Walter
dc.date.available
2015-10-05T16:15:44Z
dc.date.issued
2013-10-18
dc.identifier.citation
López Fontana, Constanza Matilde; Sasso, Corina Verónica; Maselli, María Eugenia; Santiano, Flavia Eliana; Semino, Silvana Noemi; et al.; Experimental hypothyroidism increases apoptosis in dimethylbenzanthracene-induced mammary tumors; Spandidos Publ Ltd; Oncology Reports; 30; 4; 18-10-2013; 1651-1660
dc.identifier.issn
1021-335X
dc.identifier.uri
http://hdl.handle.net/11336/2316
dc.description.abstract
Epidemiological and in vitro data have not provided conclusive evidence concerning the involvement of thyroid hormones (THs) on mammary carcinogenesis. We used an in vivo model to assess the relationship between THs, adipose tissue and breast cancer development. Female Sprague-Dawley rats were treated with a dose of 7,12-dimethylbenz(a)anthracene (15 mg/rat) at 55 days of age and were then divided into four experimental groups: hypothyroid rats (HypoT, 0.01% 6-N-propyl-2-thiouracil in drinking water), untreated control (EUT); hyperthyroid rats (HyperT, 0.25 mg/kg/day T4 s.c.) and vehicle-treated control rats. The latency of tumor appearance and the incidence and progression of tumors were determined. At sacrifice, blood samples were collected for hormone determinations and samples of tumor and mammary glands were obtained for immunohistological studies. HypoT rats had retarded growth and an increase in mammary fat. The latency was longer (p<0.0001), the incidence rate was lower (p<0.05) and tumor growth was slower in HypoT rats compared to EUT and HyperT rats. Mitotic index and PCNA immunostaining were similar in all groups. HypoT rats showed increased apoptosis (p<0.05) as evaluated by the apoptotic index and TUNEL staining. No differences in serum prolactin and progesterone were observed. However, circulating estradiol (E2) was significantly lower in HypoT and HyperT rats. Serum leptin levels were reduced in HypoT rats even though the abdominal fat mass was similar in all groups. To note, the leptin level was higher in HypoT rats that developed mammary tumors than the level in non-tumoral HypoT rats. In conclusion, hypothyroidism altered animal growth, breast morphology, body composition, leptin secretion and serum E2 enhancing apoptosis and, consequently, retarding mammary carcinogenesis in rats.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Spandidos Publ Ltd
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
APOPTOSIS
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ESTRADIOL
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HYPERTHYROIDISM
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HYPOTHYROIDISM
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LEPTIN
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Patología
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Experimental hypothyroidism increases apoptosis in dimethylbenzanthracene-induced mammary tumors
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
30
dc.journal.number
4
dc.journal.pagination
1651-1660
dc.journal.pais
Grecia
dc.journal.ciudad
Atenas
dc.description.fil
Fil: López Fontana, Constanza Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
dc.description.fil
Fil: Sasso, Corina Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
dc.description.fil
Fil: Maselli, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
dc.description.fil
Fil: Santiano, Flavia Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
dc.description.fil
Fil: Semino, Silvana Noemi. Universidad Nacional de Cuyo; Argentina
dc.description.fil
Fil: Cuello Carrión, Fernando Darío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
dc.description.fil
Fil: Jahn, Graciela Alma. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
dc.description.fil
Fil: Caron, Ruben Walter. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
dc.journal.title
Oncology Reports
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3892/or.2013.2648
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.spandidos-publications.com/or/30/4/1651
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.spandidos-publications.com/10.3892/or.2013.2648
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