Artículo
SPARC Controls Melanoma Cell Plasticity through Rac1.
Salvatierra Colussi, Edgardo Enrique
; Alvarez, Mariano J.; Leishman, Claudia C.; Rivas Baquero, Elvia Carolina
; Lutzky, Viviana P.; Chuluyan, Hector Eduardo
; Podhajcer, Osvaldo Luis
Fecha de publicación:
08/2015
Editorial:
Public Library of Science
Revista:
Plos One
ISSN:
1932-6203
e-ISSN:
1932-6203
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Cell transition to a more aggressive mesenchymal-like phenotype is a hallmark of cancer progression that involves different steps and requires tightly regulated cell plasticity. SPARC (Secreted Protein Acidic and Rich in Cysteine) is a matricellular protein that promotes this transition in various malignant cell types, including melanoma cells. We found that suppression of SPARC expression in human melanoma cells compromised cell migration, adhesion, cytoskeleton structure, and cell size. These changes involved the Akt/mTOR pathway. Re-expression of SPARC or protein addition restored all the cell features. Suppression of SPARC expression was associated with increased Rac1-GTP levels and its membrane localization. Expression of the dominant negative mutant of Rac1 counteracted almost all the changes observed in SPARC-deficient cells. Overall, these data suggest that most of the SPARC-mediated effects occurred mainly through the blockade of Rac1 activity.
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Articulos(CEFYBO)
Articulos de CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Articulos de CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Articulos(IIBBA)
Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Citación
Salvatierra Colussi, Edgardo Enrique; Alvarez, Mariano J.; Leishman, Claudia C.; Rivas Baquero, Elvia Carolina; Lutzky, Viviana P.; et al.; SPARC Controls Melanoma Cell Plasticity through Rac1.; Public Library of Science; Plos One; 10; 8; 8-2015; 1-20; e0134714
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