Artículo
Haemovigilance survey and screening strategy for arthropod-borne viruses in blood donors from Argentina
Blanco, Sebastián; Marín, Ángeles Lorena; Frutos, Maria Celia
; Yandar Barahona, Nubia Yadira
; Rivarola, María Elisa
; Carrizo, Luis Horacio; Spinsanti, Lorena Ivana; Gallego, Sandra Veronica
Fecha de publicación:
02/2024
Editorial:
Wiley-liss, div John Wiley & Sons Inc.
Revista:
Journal of Medical Virology
ISSN:
0146-6615
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Arthropod-borne viruses (arboviruses) count among emerging infections, which represent a major challenge for transfusion safety worldwide. To assess the risk of arboviruses-transmission by transfusion (ATT), we performed a survey to evaluate the potential threat for transfusion safety. Samples were retrospectively and randomly collected from donors who donated during the peak of dengue incidence in Cordoba (years: 2016 and 2019-2022). A cost-efficient strategy for molecular screening was implemented with a nucleic acid test (NAT) configured with Flavivirus and Alphavirus-universal degenerated primers targeting conserved gene regions. Besides, we evaluated the neutralizing antibody (NAb) prevalence by plaque reduction neutralization test (PRNT). A total of 1438 samples were collected. Among the NAT-screened samples, one resulted positive for Flavivirus detection. Subsequent sequencing of the PCR product revealed Saint Louis Encephalitis Virus (SLEV) infection (GeneBank accession number OR236721). NAb prevalence was 2.95% for anti-Dengue, 9.94% anti-SLEV, 1.09% anti-West Nile Virus, and 0% anti-Chikungunya. One of the NAb-positive samples also resulted positive for IgM against SLEV but negative by ARN detection. This is the first haemovigilance study developed in Argentina that evaluates the potential risk of ATT and the first research to determine the prevalence of NAb against Flavivirus through PNRT to avoid possible cross-reactions between Ab against Flavivirus. Herein, the finding of one SLEV-viremic donor and the detection of anti-SLEV IgM in a different donor demonstrated a potential threat for transfusion safety and emphasized the need for increased vigilance and proactive measures to ensure the safety of blood supplies.
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Articulos(CCT - CORDOBA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Citación
Blanco, Sebastián; Marín, Ángeles Lorena; Frutos, Maria Celia; Yandar Barahona, Nubia Yadira; Rivarola, María Elisa; et al.; Haemovigilance survey and screening strategy for arthropod-borne viruses in blood donors from Argentina; Wiley-liss, div John Wiley & Sons Inc.; Journal of Medical Virology; 96; 2; 2-2024; 1-7
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