Mostrar el registro sencillo del ítem

dc.contributor.author
Zurita, Maria Eugenia
dc.contributor.author
Moreno, Griselda Noemí
dc.contributor.author
Errea, Agustina Juliana
dc.contributor.author
Ormazabal, Maximiliano Emanuel
dc.contributor.author
Rumbo, Martín
dc.contributor.author
Hozbor, Daniela Flavia
dc.date.available
2017-08-28T14:35:41Z
dc.date.issued
2013-04
dc.identifier.citation
Zurita, Maria Eugenia; Moreno, Griselda Noemí; Errea, Agustina Juliana; Ormazabal, Maximiliano Emanuel; Rumbo, Martín; et al.; The stimulated innate resistance event in Bordetella pertussis infection is dependent on reactive oxygen species production; American Society for Microbiology; Infection and Immunity; 81; 7; 4-2013; 2371-2378
dc.identifier.issn
0019-9567
dc.identifier.uri
http://hdl.handle.net/11336/23091
dc.description.abstract
The exacerbated induction of innate immune responses in airways can abrogate diverse lung infections by a phenomenon known as stimulated innate resistance (StIR). We recently demonstrated that the enhancement of innate response activation can effi- ciently impair Bordetella pertussis colonization in a Toll-like receptor 4 (TLR4)-dependent manner. The aim of this work was to further characterize the effect of lipopolysaccharide (LPS) on StIR and to identify the mechanisms that mediate this process. Our results showed that bacterial infection was completely abrogated in treated mice when the LPS of B. pertussis (1 g) was added before (48 h or 24 h), after (24 h), or simultaneously with the B. pertussis challenge (107 CFU). Moreover, we detected that LPS completely cleared bacterial infection as soon as 2 h posttreatment. This timing suggests that the observed StIR phenomenon should be mediated by fast-acting antimicrobial mechanisms. Although neutrophil recruitment was already evident at this time point, depletion assays using an anti-GR1 antibody showed that B. pertussis clearance was achieved even in the absence of neutrophils. To evaluate the possible role of free radicals in StIR, we performed animal assays using the antioxidant N-acetyl cysteine (NAC), which is known to inactivate oxidant species. NAC administration blocked the B. pertussis clearance induced by LPS. Nitrite concentrations were also increased in the LPS-treated mice; however, the inhibition of nitric oxide synthetases did not suppress the LPS-induced bacterial clearance. Taken together, our results show that reactive oxygen species (ROS) play an essential role in the TLR4-dependent innate clearance of B. pertussis.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society for Microbiology
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Stir
dc.subject
Bordetella Pertussis
dc.subject
Tlr4
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
The stimulated innate resistance event in Bordetella pertussis infection is dependent on reactive oxygen species production
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-08-23T20:35:19Z
dc.journal.volume
81
dc.journal.number
7
dc.journal.pagination
2371-2378
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Washington
dc.description.fil
Fil: Zurita, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
dc.description.fil
Fil: Moreno, Griselda Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina
dc.description.fil
Fil: Errea, Agustina Juliana. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Ormazabal, Maximiliano Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
dc.description.fil
Fil: Rumbo, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina
dc.description.fil
Fil: Hozbor, Daniela Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
dc.journal.title
Infection and Immunity
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1128/IAI.00336-13
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://iai.asm.org/content/81/7/2371


Archivos asociados

 

Comunidades y colecciones

Mostrar el registro sencillo del ítem