Artículo
Extra-virgin olive oil ameliorates high-fat diet-induced seminal and testicular disorders by modulating the cholesterol pathway
Funes, Abi Karenina
; Avena Alvarez, María Virginia
; Ibañez, Jorge Ernesto
; Simón, Layla Yamila
; Ituarte, Leonor María Ester; Colombo, Regina Lucía
; Roldán, Adrián; Conte, María Inés
; Monclus, Maria de Los Angeles
; Boarelli, Paola Vanina
; Fornes, Miguel Walter
; Saez Lancellotti, Tania Emilce Estefania
Fecha de publicación:
01/2023
Editorial:
John Wiley & Sons
Revista:
Andrology
ISSN:
2047-2919
e-ISSN:
2047-2927
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Background: Rabbits are sensitive to dietary cholesterol and rapidly develop hypercholesterolemia, leading to prominent subfertility. Sterol regulatory element-binding protein isoform 2 drives the intracellular cholesterol pathway in many tissues, including the testicles. Its abnormal regulation could be the mainly responsible for the failure of suppressing cholesterol synthesis in a cholesterol-enriched environment, ultimately leading to testicular and seminal alterations. However, extra-virgin olive oil consumption has beneficial properties that promote lowering of cholesterol levels, including the recovery of seminal parameters altered under a high-fat diet. Objectives: Our goal was to investigate the effects of high-fat diet supplementation with extra-virgin olive oil at the molecular level on rabbit testes, by analyzing sterol regulatory element-binding protein isoform 2 protein and its corresponding downstream effectors. Materials and methods: During 12 months, male rabbits were fed a control diet, high-fat diet, or 6-month high-fat diet followed by 6-month high-fat diet plus extra-virgin olive oil. Serum lipids, testosterone levels, bodyweight, and seminal parameters were tested. The mRNA and protein levels of sterol regulatory element-binding protein isoform 2, 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase, and low-density lipoprotein receptor were determined by semi-quantitative polymerase chain reaction and Western blotting techniques. The expression pattern of sterol regulatory element-binding protein isoform 2 protein in the rabbit testicles was studied by indirect immunofluorescence. In addition, testicular cholesterol was detected and quantified by filipin staining and gas chromatography. Results: The data showed that the addition of extra-virgin olive oil to high-fat diet reduced testicular cholesterol levels and recovered the expression of sterol regulatory element-binding protein isoform 2, 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase, and low-density lipoprotein receptor initially altered by the high-fat diet. Discussion and conclusions: The combination of high-fat diet with extra-virgin olive oil encourages testicular recovery by modifying the expression of the enzymes related to intracellular cholesterol management.
Palabras clave:
CHOLESTEROL
,
MALE INFERTILITY
,
OLIVE OIL
,
SPERMATOZOA
,
SREBP
,
TESTICLE
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Articulos(IHEM)
Articulos de INST. HISTOLOGIA Y EMBRIOLOGIA DE MEND DR.M.BURGOS
Articulos de INST. HISTOLOGIA Y EMBRIOLOGIA DE MEND DR.M.BURGOS
Citación
Funes, Abi Karenina; Avena Alvarez, María Virginia; Ibañez, Jorge Ernesto; Simón, Layla Yamila; Ituarte, Leonor María Ester; et al.; Extra-virgin olive oil ameliorates high-fat diet-induced seminal and testicular disorders by modulating the cholesterol pathway; John Wiley & Sons; Andrology; 11; 6; 1-2023; 1203-1217
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