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dc.contributor.author
Carossino, Mariano
dc.contributor.author
Balasuriya, Udeni B. R.
dc.contributor.author
Thieulent, Come J.
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Barrandeguy, Maria E.
dc.contributor.author
Vissani, María Aldana
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dc.contributor.author
Parreño, Gladys Viviana
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dc.date.available
2024-03-19T10:16:53Z
dc.date.issued
2023-06
dc.identifier.citation
Carossino, Mariano; Balasuriya, Udeni B. R.; Thieulent, Come J.; Barrandeguy, Maria E.; Vissani, María Aldana; et al.; Quadruplex Real-Time TaqMan® RT-qPCR Assay for Differentiation of Equine Group A and B Rotaviruses and Identification of Group A G3 and G14 Genotypes; MDPI; Viruses; 15; 8; 6-2023; 1-16
dc.identifier.issn
1999-4915
dc.identifier.uri
http://hdl.handle.net/11336/230839
dc.description.abstract
Equine rotavirus A (ERVA) is the leading cause of diarrhea in foals, with G3P[12] and G14P[12] genotypes being the most prevalent. Recently, equine G3-like RVA was recognized as an emerging infection in children, and a group B equine rotavirus (ERVB) was identified as an emergent cause of foal diarrhea in the US. Thus, there is a need to adapt molecular diagnostic tools for improved detection and surveillance to identify emerging strains, understand their molecular epidemiology, and inform future vaccine development. We developed a quadruplex TaqMan® RT-qPCR assay for differentiation of ERVA and ERVB and simultaneous G-typing of ERVA strains, evaluated its analytical and clinical performance, and compared it to (1) a previously established ERVA triplex RT-qPCR assay and (2) standard RT-PCR assay and Sanger sequencing of PCR products. This quadruplex RT-qPCR assay demonstrated high sensitivity (>90%)/specificity (100%) for every target and high overall agreement (>96%). Comparison between the triplex and quadruplex assays revealed only a slightly higher sensitivity for the ERVA NSP3 target using the triplex format (p-value 0.008) while no significant differences were detected for other targets. This quadruplex RT-qPCR assay will significantly enhance rapid surveillance of both ERVA and ERVB circulating and emerging strains with potential for interspecies transmission.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
MDPI
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
ERVA
dc.subject
Molecular diagnosis
dc.subject
Real time PCR
dc.subject
ERVB
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Otras Ciencias Veterinarias
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dc.subject.classification
Ciencias Veterinarias
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CIENCIAS AGRÍCOLAS
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dc.title
Quadruplex Real-Time TaqMan® RT-qPCR Assay for Differentiation of Equine Group A and B Rotaviruses and Identification of Group A G3 and G14 Genotypes
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-03-18T11:23:22Z
dc.journal.volume
15
dc.journal.number
8
dc.journal.pagination
1-16
dc.journal.pais
Suiza
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dc.description.fil
Fil: Carossino, Mariano. State University of Louisiana; Estados Unidos
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Fil: Balasuriya, Udeni B. R.. State University of Louisiana; Estados Unidos
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Fil: Thieulent, Come J.. State University of Louisiana; Estados Unidos
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Fil: Barrandeguy, Maria E.. Universidad del Salvador; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
dc.description.fil
Fil: Vissani, María Aldana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Universidad del Salvador; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Viruses
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3390/v15081626
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