Artículo
Enhanced recombinant protein capture, purity and yield from crude bacterial cell extracts by N-Lauroylsarcosine-assisted affinity chromatography
Vicente Carratalá, José; Atienza Garriga, Jan; López Laguna, Héctor; Vázquez, Esther; Villaverde, Antonio; Sanchez, Julieta Maria
; Ferrer Miralles, Neus
Fecha de publicación:
04/2023
Editorial:
BioMed Central
Revista:
Microbial Cell Factories
ISSN:
1475-2859
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Background Recombinant proteins cover a wide range of biomedical, biotechnological, and industrial needs.Although there are diverse available protocols for their purification from cell extracts or from culture media, many pro‑teins of interest such as those containing cationic domains are difficult to purify, a fat that results in low yields of thefinal functional product. Unfortunately, this issue prevents the further development and industrial or clinical applica‑tion of these otherwise interesting products.Results Aiming at improving the purification of such difficult proteins, a novel procedure has been developed basedon supplementing crude cell extracts with non-denaturing concentrations of the anionic detergent N-Lauroylsar‑cosine. The incorporation of this simple step in the downstream pipeline results in a substantial improvement of theprotein capture by affinity chromatography, an increase of protein purity and an enhancement of the overall processyield, being the detergent not detectable in the final product.Conclusion By taking this approach, which represents a smart repurposing of N-Lauroylsarcosine applied to proteindownstream, the biological activity of the protein is not affected. Being technologically simple, the N-Lauroylsarco‑sine-assisted protein purification might represent a critical improvement in recombinant protein production withwide applicability, thus smothering the incorporation of promising proteins into the protein market.
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Articulos(IIBYT)
Articulos de INSTITUTO DE INVESTIGACIONES BIOLOGICAS Y TECNOLOGICAS
Articulos de INSTITUTO DE INVESTIGACIONES BIOLOGICAS Y TECNOLOGICAS
Citación
Vicente Carratalá, José; Atienza Garriga, Jan; López Laguna, Héctor; Vázquez, Esther; Villaverde, Antonio; et al.; Enhanced recombinant protein capture, purity and yield from crude bacterial cell extracts by N-Lauroylsarcosine-assisted affinity chromatography; BioMed Central; Microbial Cell Factories; 22; 1; 4-2023; 1-14
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