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Artículo

Identification of Ensifer meliloti genes required for survival during peat-based bioinoculant maturation by STM-seq

Lozano, Mauricio JavierIcon ; Mogro, Ezequiel GerardoIcon ; Salas, María EugeniaIcon ; Erdozain, Sofía A.; Zuber, Nicolás EmilioIcon ; Becker, Anke; Lagares, AntonioIcon
Fecha de publicación: 01/2023
Editorial: Elsevier Science
Revista: Journal of Biotechnology
ISSN: 0168-1656
e-ISSN: 1873-4863
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biología Celular, Microbiología

Resumen

Rhizobial inoculants are sold either as rhizobia within a liquid matrix; or as rhizobia adhered to granules composed of peat prill or finely ground peat moss. During the production of peat-based inoculants, a series of physiological changes occur that result in an increased capability of the rhizobia to survive on the seeds. The number of viable rhizobia on preinoculated seeds at the point of sale, however, is often a limiting factor, as is the inefficiency of the inoculant bacteria to compete with the local rhizobia for the host colonization. In the present work, we used STM-seq for the genome-wide screening of Ensifer meliloti mutants affected in the survival during the maturation of peat-based inoculant formulations. Through this approach, we were able to identify a set of mutants whose behavior suggests that persistence in peat inoculants involves a complex phenotype that is connected to diverse cellular activities, mainly related to satisfying the requirements of bacterial nutrition (e.g., carbon sources, ions) and to coping with specific stresses (e.g., oxidative, mutational). These results also provide a base knowledge that could be used to more completely understand the survival mechanisms used by rhizobia during the maturation of peat-based inoculants, as well as for the design and implementation of practical strategies to improve inoculant formulations.
Palabras clave: INOCULANT , PEAT , PHENOMICS , RHIZOBIUM , SIGNATURE TAGGED MUTAGENESIS
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)
Identificadores
URI: http://hdl.handle.net/11336/230159
DOI: http://dx.doi.org/10.1016/j.jbiotec.2022.12.004
URL: https://www.sciencedirect.com/science/article/pii/S0168165622002917
Colecciones
Articulos(IBBM)
Articulos de INST.DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Citación
Lozano, Mauricio Javier; Mogro, Ezequiel Gerardo; Salas, María Eugenia; Erdozain, Sofía A.; Zuber, Nicolás Emilio; et al.; Identification of Ensifer meliloti genes required for survival during peat-based bioinoculant maturation by STM-seq; Elsevier Science; Journal of Biotechnology; 362; 1-2023; 12-23
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