Artículo
The Exosome-like Vesicles of Giardia Assemblages A, B, and E Are Involved in the Delivering of Distinct Small RNA from Parasite to Parasite
Natali, Lautaro
; Luna Pizarro, Gabriel; Moyano, Sofia
; de la Cruz Thea, Benjamín Isaías
; Musso, Juliana Rita; Ropolo, Andrea Silvana
; Eichner, Norbert; Meister, Gunter; Musri, Melina Mara
; Feliziani, Constanza
; Touz, Maria Carolina
Fecha de publicación:
05/2023
Editorial:
Molecular Diversity Preservation International
Revista:
International Journal of Molecular Sciences
ISSN:
1422-0067
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The genetically related assemblages of the intestinal protozoa parasite Giardia lamblia are morphologically indistinguishable and are often derived from specific hosts. The Giardia assemblages are separated by large genetic distances, which might account for their relevant biological and pathogenic differences. In this work, we analyzed the RNAs cargo released into exosomal-like vesicles (ElVs) by the assemblages A and B, which differentially infect humans, and the assemblage E, which infects hoofed animals. The RNA sequencing analysis revealed that the ElVs of each assemblage contained distinct small RNA (sRNA) biotypes, suggesting a preference for specific packaging in each assemblage. These sRNAs were classified into three categories, ribosomal-small RNAs (rsRNAs), messenger-small RNAs (msRNAs), and transfer-small RNAs (tsRNAs), which may play a regulatory role in parasite communication and contribute to host-specificity and pathogenesis. Uptake experiments showed, for the first time, that ElVs were successfully internalized by the parasite trophozoites. Furthermore, we observed that the sRNAs contained inside these ElVs were first located below the plasma membrane but then distributed along the cytoplasm. Overall, the study provides new insights into the molecular mechanisms underlying the host-specificity and pathogenesis of G. lamblia and highlights the potential role of sRNAs in parasite communication and regulation.
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(INIMEC - CONICET)
Articulos de INSTITUTO DE INV. MEDICAS MERCEDES Y MARTIN FERREYRA
Articulos de INSTITUTO DE INV. MEDICAS MERCEDES Y MARTIN FERREYRA
Citación
Natali, Lautaro; Luna Pizarro, Gabriel; Moyano, Sofia; de la Cruz Thea, Benjamín Isaías; Musso, Juliana Rita; et al.; The Exosome-like Vesicles of Giardia Assemblages A, B, and E Are Involved in the Delivering of Distinct Small RNA from Parasite to Parasite; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 24; 11; 5-2023; 1-14
Compartir
Altmétricas