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dc.contributor.author
Flores Bragulat, Ana Paula
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dc.contributor.author
Ortiz, Isabel
dc.contributor.author
Catalán, Jaime
dc.contributor.author
Dorado, Jesus
dc.contributor.author
Hidalgo, Manuel
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Losinno, Luis
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dc.contributor.author
Dordas Perpinyà, Marta
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Yánez Ortiz, Iván
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Martínez Rodero, Iris
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Miró, Jordi
dc.contributor.author
Gambini, Andres
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dc.date.available
2024-03-11T11:18:17Z
dc.date.issued
2023-01
dc.identifier.citation
Flores Bragulat, Ana Paula; Ortiz, Isabel; Catalán, Jaime; Dorado, Jesus; Hidalgo, Manuel; et al.; Time-lapse imaging and developmental competence of donkey eggs after ICSI: Effect of preovulatory follicular fluid during oocyte in vitro maturation; Elsevier Science Inc.; Theriogenology; 195; 1-2023; 199-208
dc.identifier.issn
0093-691X
dc.identifier.uri
http://hdl.handle.net/11336/229956
dc.description.abstract
Equus members exhibit very divergent karyotype, genetic plasticity, and significant differences in their reproductive physiology. Despite the fact that somatic cell nuclear transfer and intracytoplasmic sperm injection (ICSI) has gained relevance in the last few years in horses, few reports have been published exploring ovum pick up (OPU) and in vitro maturation (IVM) of cumulus-oocyte complexes (COCs) in donkeys. Yet, some donkey species and breeds are considered endangered, and these assisted-reproductive technologies could help to preserve the genetic of valuable individuals. In this study, we tested the hypothesis that supplementation with jenny preovulatory follicular fluid (PFF) during IVM could improve oocyte developmental competence in the donkey. For this, in vitro nuclear maturation rates, cumulus cell expansion, and embryo development after ICSI of donkey COCs matured in culture media supplemented with fetal bovine serum (FBS) or donkey PFF, with a known metabolomic profile, were assessed. Time-lapse imagining was performed after ICSI of horse and donkey oocytes. Eight OPU sessions were done in five jennies with an average recovery rate of 69.2% (n = 45 COCs). Although lower cumulus cells expansion was observed in oocytes of PFF group (P = 0.0010), no significant differences were described in nuclear maturation rates and preimplantation embryo development between groups. Donkey ICSI embryos showed similar morphokinetics to horse ICSI embryos. Our study shows that supplementing IVM media with FBS or donkey PFF supports nuclear maturation and early preimplantation embryo development after ICSI in donkeys. To our knowledge, the present study is the first report of ICSI, time-lapse imaging and in vitro blastocyst production in donkey.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science Inc.
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dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ASSES GENETIC PRESERVATION
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EQUUS WILD CONSERVATION
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IN VITRO EMBRYO PRODUCTION
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METABOLOMICS
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SPERM INJECTION
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TRANSVAGINAL OOCYTE ASPIRATION
dc.subject.classification
Otras Ciencias Biológicas
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Time-lapse imaging and developmental competence of donkey eggs after ICSI: Effect of preovulatory follicular fluid during oocyte in vitro maturation
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-03-08T14:47:10Z
dc.journal.volume
195
dc.journal.pagination
199-208
dc.journal.pais
Estados Unidos
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dc.description.fil
Fil: Flores Bragulat, Ana Paula. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Producción Animal. Laboratorio de Reproducción Equina; Argentina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Instituto de Ciencias Veterinarias. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Ciencias Veterinarias.; Argentina
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Fil: Ortiz, Isabel. Universidad de Córdoba; España
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Fil: Catalán, Jaime. Universitat Autònoma de Barcelona; España
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Fil: Dorado, Jesus. Universidad de Córdoba; España
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Fil: Hidalgo, Manuel. Universidad de Córdoba; España
dc.description.fil
Fil: Losinno, Luis. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Departamento de Producción Animal. Laboratorio de Reproducción Equina; Argentina
dc.description.fil
Fil: Dordas Perpinyà, Marta. Universitat Autònoma de Barcelona; España
dc.description.fil
Fil: Yánez Ortiz, Iván. Universitat Autònoma de Barcelona; España
dc.description.fil
Fil: Martínez Rodero, Iris. Universitat Autònoma de Barcelona; España
dc.description.fil
Fil: Miró, Jordi. Universitat Autònoma de Barcelona; España
dc.description.fil
Fil: Gambini, Andres. Universidad de Córdoba; España. University of Queensland; Australia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Theriogenology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.theriogenology.2022.10.030
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