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dc.contributor.author
Niu, Shuang
dc.contributor.author
Shingle, Danielle L.
dc.contributor.author
Garbarino Pico, Eduardo
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dc.contributor.author
Kojima, Shihoko
dc.contributor.author
Gilbert, Misty
dc.contributor.author
Green, Carla B.
dc.date.available
2024-03-08T10:29:47Z
dc.date.issued
2011-11
dc.identifier.citation
Niu, Shuang; Shingle, Danielle L.; Garbarino Pico, Eduardo; Kojima, Shihoko; Gilbert, Misty; et al.; The circadian deadenylase nocturnin is necessary for stabilization of the iNOS mRNA in mice; Public Library of Science; Plos One; 6; 11; 11-2011; 26954-26963
dc.identifier.issn
1932-6203
dc.identifier.uri
http://hdl.handle.net/11336/229758
dc.description.abstract
Nocturnin is a member of the CCR4 deadenylase family, and its expression is under circadian control with peak levels at night. Because it can remove poly(A) tails from mRNAs, it is presumed to play a role in post-transcriptional control of circadian gene expression, but its target mRNAs are not known. Here we demonstrate that Nocturnin expression is acutely induced by the endotoxin lipopolysaccharide (LPS). Mouse embryo fibroblasts (MEFs) lacking Nocturnin exhibit normal patterns of acute induction of TNFa and iNOS mRNAs during the first three hours following LPS treatment, but by 24 hours, while TNFa mRNA levels are indistinguishable from WT cells, iNOS message is significantly reduced 20-fold. Accordingly,analysis of the stability of the mRNAs showed that loss of Nocturnin causes a significant decrease in the half-life of the iNOS mRNA (t1/2 = 3.3 hours in Nocturnin knockout MEFs vs. 12.4 hours in wild type MEFs), while having no effect on the TNFa message. Furthermore, mice lacking Nocturnin lose the normal nighttime peak of hepatic iNOS mRNA, and have improved survival following LPS injection. These data suggest that Nocturnin has a novel stabilizing activity that plays an important role in the circadian response to inflammatory signals.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library of Science
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Nocturnin
dc.subject
iNOS
dc.subject
Circadian Rhythms
dc.subject
Posttranscriptional regulation
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
The circadian deadenylase nocturnin is necessary for stabilization of the iNOS mRNA in mice
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-03-05T13:23:20Z
dc.journal.volume
6
dc.journal.number
11
dc.journal.pagination
26954-26963
dc.journal.pais
Estados Unidos
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dc.description.fil
Fil: Niu, Shuang. University of Virginia; Estados Unidos
dc.description.fil
Fil: Shingle, Danielle L.. University of Virginia; Estados Unidos. University of Texas; Estados Unidos
dc.description.fil
Fil: Garbarino Pico, Eduardo. University of Virginia; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
dc.description.fil
Fil: Kojima, Shihoko. University of Virginia; Estados Unidos. University of Texas; Estados Unidos
dc.description.fil
Fil: Gilbert, Misty. University of Virginia; Estados Unidos
dc.description.fil
Fil: Green, Carla B.. University of Virginia; Estados Unidos. University of Texas; Estados Unidos
dc.journal.title
Plos One
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0026954
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0026954
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