Artículo
Structure of a lectin with antitumoral properties in king bolete (Boletus edulis) mushrooms
Bovi, Michele; Carrizo, Maria Eugenia
; Capaldi, Stefano; Perduca, Massimiliano; Chiarelli, Laurent; Galliano, Monica; Monaco, Hugo
Fecha de publicación:
08/2011
Editorial:
Oxford University Press
Revista:
Glycobiology
ISSN:
0959-6658
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
A novel lectin has been isolated from the fruiting bodies of the common edible mushroom Boletus edulis (king bolete, penny bun, porcino, or cep) by affinity chromatography on a chitin column. We propose for the lectin the name BEL (Boletus edulis lectin). BEL inhibits selectively the proliferation of several malignant cell lines and binds the neoplastic cell specific T-antigen disaccharide, Galbeta1-3GalNAc. The lectin was structurally characterized: the molecule is a homotetramer and the 142 amino acid sequence of the chains was determined. The protein belongs to the saline-soluble family of mushroom fruiting body specific lectins. BEL was also crystallized and its three-dimensional structure was determined by X-ray diffraction to 1.15 Å resolution. The structure is similar to that of Agaricus bisporus lectin. Using the appropriate co-crystals, the interactions of BEL with specific mono and disaccharides were also studied by X-ray diffraction. The six structures of carbohydrate complexes reported here provide details of the interactions of the ligands with the lectin and shed light on the selectivity of the two distinct binding sites present in each protomer.
Palabras clave:
MUSHROOM
,
LECTIN
,
STRUCTURE
,
T-ANTIGEN
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Licencia
Identificadores
Colecciones
Articulos(CIQUIBIC)
Articulos de CENTRO DE INVEST.EN QCA.BIOL.DE CORDOBA (P)
Articulos de CENTRO DE INVEST.EN QCA.BIOL.DE CORDOBA (P)
Citación
Bovi, Michele; Carrizo, Maria Eugenia; Capaldi, Stefano; Perduca, Massimiliano; Chiarelli, Laurent; et al.; Structure of a lectin with antitumoral properties in king bolete (Boletus edulis) mushrooms; Oxford University Press; Glycobiology; 21; 8; 8-2011; 1000-1009
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