Artículo
Optimization and comparative analysis of LAMP and PCR techniques for the detection of leptospiral DNA in Golden Syrian hamsters
Hamer, Micaela
; Watanabe, Olivia
; Saraullo, Vanina Rosa
; Ortega, Facundo; Sánchez, Cristina; Martínez, Mara; Brihuega, Bibiana; Grune Loffler, Sylvia
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Fecha de publicación:
08/2023
Editorial:
Springer
Revista:
Veterinary Research Communications
ISSN:
0165-7380
e-ISSN:
1573-7446
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Leptospirosis is a zoonotic disease with significant public health and economic impact worldwide. Rapid and accurate diagnosis is essential for effective prevention and treatment. This study optimized a loop-mediated isothermal amplification (LAMP) assay using BFo isothermal DNA polymerase with different colorimetric indicators. LAMP was able to detect DNA from pathogenic and intermediate leptospires, while non-pathogenic leptospires and other non-leptospiral microorganisms were negative. LAMP assay combined with calcein showed a tenfold higher limit of detection (1 ng of leptospiral DNA per reaction) than LAMP combined with hydroxynaphthol blue or end-point PCR lipL32 (10 ng of DNA per reaction). Animal samples were collected from infected and non-infected Golden Syrian hamsters (Mesocricetus auratus) to evaluate and compare the performance of LAMP and PCR. These techniques showed a substantial agreement according to Cohen’s kappa statistic, being both useful techniques for detecting leptospiral DNA in clinical samples. Overall, this study demonstrates that the LAMP assay is a sensitive, specific, rapid, and simple tool for the detection of leptospiral DNA. It has the potential to facilitate the diagnosis of leptospirosis, particularly in low-income regions with limited diagnosis resources.
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Articulos (IPVET)
Articulos de INSTITUTO DE PATOBIOLOGIA VETERINARIA
Articulos de INSTITUTO DE PATOBIOLOGIA VETERINARIA
Citación
Hamer, Micaela; Watanabe, Olivia; Saraullo, Vanina Rosa; Ortega, Facundo; Sánchez, Cristina; et al.; Optimization and comparative analysis of LAMP and PCR techniques for the detection of leptospiral DNA in Golden Syrian hamsters; Springer; Veterinary Research Communications; 48; 1; 8-2023; 103-111
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