Mostrar el registro sencillo del ítem
dc.contributor.author
Demonte, Luisina Delma
dc.contributor.author
Magni, Florencia Valentina
dc.contributor.author
Michlig, Melina Paola
dc.contributor.author
Michlig, Nicolás
dc.contributor.author
Repetti, María Rosa
dc.date.available
2024-02-28T10:52:15Z
dc.date.issued
2021
dc.identifier.citation
Determination of glyphosate, AMPA and glufosinate in honey: Comparative tests on the sample preparation stage; 8th Latin American Pesticide Residue Workshop; Panamá; Panamá; 2021; 42-42
dc.identifier.uri
http://hdl.handle.net/11336/228684
dc.description.abstract
Beekeeping is an activity of great economic and eco-systemic importance, and Argentina is a major country in the world in this activity. Regarding the cleaning and safety of honey, residues and chemical contaminants´ unwanted presence is of great concern, both by residues originated in the treatments applied to the hive and by the use of agrochemicals in agriculture that potentially contaminate the beekeeping chain. To meet today´s quality and safety requirements is necessary to realize more pesticide control analysis.Argentina, The United States and Brazil use 80% of glyphosate´s world total. In our country since 1996 has been applied to various transgenic crops headed by soy, with a significant annual load (200 thousand tons). Therefore, the main objective was to develop an analytical methodology to determine glyphosate, AMPA and glufosinate in honey and related matrices such as nectar. Based on the pre-column derivatization strategy with FMOC-Cl and analysis by UHPLC-MS/MS. Different alternatives were evaluated and adapted to the objective. For methodology optimization: extraction solvents, volume and concentration of the derivatizing reagent, volume and concentration of the borate buffer, different reagents to achieve the optimum pH (pH 9), reaction time and cleaning alternatives of the extracts also were evaluated (L-L partition and SPE).Recovery tests at two concentration levels (10 and 100 ug kg-1) in triplicate were performed to evaluate the methodology. First, honey samples weighed in 50 mL centrifuge tubes were placed in a thermostated bath at 40-50°C for 5 min to achieve fluidity. They were then spiked with the standard mixture (glyphosate, AMPA and glufosinate) and vortexed to homogenize. After that, the extraction, derivatization and cleaning stage were performed for each of the evaluated alternatives. The final methodology involves an extraction step with water and mechanical agitation for 30 min followed by the derivatization reaction and finally L-L partition with dichloromethane as a clean-up stage1. Satisfactory results have been obtained, showing that this method is consistent and reliable, with RSDs < 20% and recoveries between 70-105% at 5, 10, 25 and 50 ug kg-1.Also, together with the optimization of the sample preparation, to achieve a better response of the analytes as a whole, the instrumental conditions were optimized based on experiments´ statistical designs to increase detection sensitivity to obtain low detection and quantification limits.In this way, it was possible to achieve an acceptable analytical method for determining glyphosate, AMPA and glufosinate that was applied to analyze raw and commercial honey samples.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Gobierno de la República de Panamá. Ministerio de Desarrollo Agropecuario
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
GLYPHOSATE
dc.subject
HONEY
dc.subject
CHROMATOGRAPHY
dc.subject
SPECTROMETRY
dc.subject.classification
Química Analítica
dc.subject.classification
Ciencias Químicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Determination of glyphosate, AMPA and glufosinate in honey: Comparative tests on the sample preparation stage
dc.type
info:eu-repo/semantics/publishedVersion
dc.type
info:eu-repo/semantics/conferenceObject
dc.type
info:ar-repo/semantics/documento de conferencia
dc.date.updated
2022-11-09T16:52:06Z
dc.journal.pagination
42-42
dc.journal.pais
Panamá
dc.journal.ciudad
Panamá
dc.description.fil
Fil: Demonte, Luisina Delma. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
dc.description.fil
Fil: Magni, Florencia Valentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; Argentina
dc.description.fil
Fil: Michlig, Melina Paola. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
dc.description.fil
Fil: Michlig, Nicolás. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; Argentina
dc.description.fil
Fil: Repetti, María Rosa. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; Argentina
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://heyzine.com/flip-book/7316f9aa59.html#page/12
dc.conicet.rol
Autor
dc.conicet.rol
Autor
dc.conicet.rol
Autor
dc.conicet.rol
Autor
dc.conicet.rol
Autor
dc.coverage
Internacional
dc.type.subtype
Workshop
dc.description.nombreEvento
8th Latin American Pesticide Residue Workshop
dc.date.evento
2021-05-18
dc.description.ciudadEvento
Panamá
dc.description.paisEvento
Panamá
dc.type.publicacion
Book
dc.description.institucionOrganizadora
Universidad Nacional del Litoral
dc.description.institucionOrganizadora
Gobierno de la República de Panamá. Ministerio de Desarrollo Agropecuario
dc.description.institucionOrganizadora
Sociedad Latinoamericana sobre Residuos de Plaguicidas
dc.source.libro
Book of Abstracts: 8th Latinamerican Pesticide Residues Workshop LAPRW 2021. Food and Environment
dc.date.eventoHasta
2021-05-20
dc.type
Workshop
Archivos asociados