Artículo
Characterization of cell-to-cell variation in nuclear transport rates and identification of its sources
Durrieu, Lucía
; Bush, Alan
; Grande, Alicia Viviana
; Rikard, Johansson; David, Janzén; Katz, Andrea; Cedersund, Gunnar; Colman Lerner, Alejandro Ariel
Fecha de publicación:
01/2023
Editorial:
Elsevier
Revista:
iScience
ISSN:
2589-0042
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Nuclear transport is an essential part of eukaryotic cell function. Here, we present scFRAP, a model-assisted fluorescent recovery after photobleaching (FRAP)- based method to determine nuclear import and export rates independently in individual live cells. To overcome the inherent noise of single-cell measurements, we performed sequential FRAPs on the same cell. We found large cell-to-cell variation in transport rates within isogenic yeast populations. For passive transport, the variability in NPC number might explain most of the variability. Using this approach, we studied mother-daughter cell asymmetry in the active nuclear shuttling of the transcription factor Ace2, which is specifically concentrated in daughter cell nuclei in early G1. Rather than reduced export in the daughter cell, as previously hypothesized, we found that this asymmetry is mainly due to an increased import in daughters. These results shed light on cell-to-cell variation in cellular dynamics and its sources.
Palabras clave:
BIOLOGICAL SCIENCES
,
CELL BIOLOGY
,
MATHEMATICAL BIOSCIENCES
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Articulos(IFIBYNE)
Articulos de INST.DE FISIOL., BIOL.MOLECULAR Y NEUROCIENCIAS
Articulos de INST.DE FISIOL., BIOL.MOLECULAR Y NEUROCIENCIAS
Citación
Durrieu, Lucía; Bush, Alan; Grande, Alicia Viviana; Rikard, Johansson; David, Janzén; et al.; Characterization of cell-to-cell variation in nuclear transport rates and identification of its sources; Elsevier; iScience; 26; 1; 1-2023; 1-20
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