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Artículo

Development of Nanobody-Displayed Whole-Cell Biosensors for the Colorimetric Detection of SARS-CoV-2

He, Yawen; Xu, Zhiyuan; Kasputis, Tom; Zhao, Xue; Ibañez, Lorena ItatíIcon ; Pavan, Maria FlorenciaIcon ; Bok, MarinaIcon ; Malito, Juan Pablo Alfonso; Parreno, Viviana; Yuan, Lijuan; Wright, R. Clay; Chen, Juhong
Fecha de publicación: 08/2023
Editorial: American Chemical Society
Revista: ACS Applied Materials & Interfaces
ISSN: 1944-8244
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Químicas

Resumen

The accurate and effective detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential to preventing the spread of infectious diseases and ensuring human health. Herein, a nanobody-displayed whole-cell biosensor was developed for colorimetric detection of SARS-CoV-2 spike proteins. Serving as bioreceptors, yeast surfaces were genetically engineered to display SARS-CoV-2 binding of llama-derived single-domain antibodies (nanobodies) with high capture efficiency, facilitating the concentration and purification of SARS-CoV-2. Gold nanoparticles (AuNPs) employed as signal transductions were functionalized with horseradish peroxidase (HRP) and anti-SARS monoclonal antibodies to enhance the detection sensitivity. In the presence of SARS-CoV-2 spike proteins, the sandwiched binding will be formed by linking engineered yeast, SARS-CoV-2 spike proteins, and reporter AuNPs. The colorimetric signal was generated by the enzymatic reaction of HRP and its corresponding colorimetric substrate/chromogen system. At the optimal conditions, the developed whole-cell biosensor enables the sensitive detection of SARS-CoV-2 spike proteins in a linear range from 0.01 to 1 μg/mL with a limit of detection (LOD) of 0.037 μg/mL (about 4 × 108 virion particles/mL). Furthermore, the whole-cell biosensor was demonstrated to detect the spike protein of different SARS-CoV-2 variants in human serum, providing new possibilities for the detection of future SARS-CoV-2 variants.
Palabras clave: COLORIMETRIC DETECTION , ENGINEERED YEAST , GOLD NANOPARTICLE , NANOBODY , SARS-COV-2 , WHOLE-CELL BIOSENSOR , COVID-19
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/228359
DOI: http://dx.doi.org/10.1021/acsami.3c05900
Colecciones
Articulos(INQUIMAE)
Articulos de INST.D/QUIM FIS D/L MATERIALES MEDIOAMB Y ENERGIA
Citación
He, Yawen; Xu, Zhiyuan; Kasputis, Tom; Zhao, Xue; Ibañez, Lorena Itatí; et al.; Development of Nanobody-Displayed Whole-Cell Biosensors for the Colorimetric Detection of SARS-CoV-2; American Chemical Society; ACS Applied Materials & Interfaces; 15; 31; 8-2023; 37184-37192
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