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dc.contributor.author
Chrestia, Juan Facundo  
dc.contributor.author
Esandi, María del Carmen  
dc.contributor.author
Bouzat, Cecilia Beatriz  
dc.date.available
2024-02-26T10:00:36Z  
dc.date.issued
2022  
dc.identifier.citation
Modulation of alpha7 nicotinic acetylcholine receptor by cannabidiol; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología & 3er Congreso Franco Argentino de Inmunología; Reunión Anual 2022 de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2022; 224-224  
dc.identifier.issn
0025-7680  
dc.identifier.uri
http://hdl.handle.net/11336/228263  
dc.description.abstract
Cannabidiol (CBD), an important terpenoid compound from marijuana with no psychoactive effects, has become of great pharmaceutical interest for several health conditions. As CBD is a multitarget drug, there is a need to establish the molecular mechanisms by which CBD may exert therapeutic as well as adverse effects. The α7 nicotinic acetylcholine receptor is a cation-permeable ACh-gated channel present in the nervous system and in non-neuronal cells. It is involved in different pathological conditions, including neurological and neurodegenerative disorders, inflammation, and cancer. The aim of this work is to study α7 as one of the therapeutic targets of CBD in order to determine the mechanism by which the phytocannabinoid exerts its effect on the receptor. Wild-type and mutant α7 receptors were expressed in mammalian cells. To evaluate receptor ionotropic function, high-resolution single channel recordings in cell-attached and inside-out patch configurations were used. Changes in intracellular calcium levels were measured by confocal microscopy. We here reveal that CBD modulates α7 ionotropic and metabotropic functions. CBD leads to a concentration-dependent decrease of α7 single-channel activity with an IC50 of 0.54 ± 0.13 µM. The inhibition of α7 activity, which takes place through a membrane pathway, is neither mediated by receptor phosphorylation nor overcome by positive allosteric modulators and is compatible with CBD stabilization of resting or desensitized α7 conformational states. At the cellular level, CBD inhibits the increase in intracellular calcium triggered by α7 activation, thus decreasing cell calcium responses. The modulation of α7 is of pharmacological relevance and should be considered in the evaluation of CBD potential therapeutic uses. Thus, our study provides novel molecular information of CBD multiple actions and targets, which is required to set the basis for prospective applications in human health.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Fundación Revista Medicina  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
NICOTINIC RECEPTORS  
dc.subject
CANNABIDIOL  
dc.subject.classification
Bioquímica y Biología Molecular  
dc.subject.classification
Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
Modulation of alpha7 nicotinic acetylcholine receptor by cannabidiol  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.type
info:eu-repo/semantics/conferenceObject  
dc.type
info:ar-repo/semantics/documento de conferencia  
dc.date.updated
2024-02-22T14:00:58Z  
dc.identifier.eissn
1669-9106  
dc.journal.volume
82  
dc.journal.number
Suplemento V  
dc.journal.pagination
224-224  
dc.journal.pais
Argentina  
dc.journal.ciudad
Buenos Aires  
dc.description.fil
Fil: Chrestia, Juan Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina  
dc.description.fil
Fil: Esandi, María del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina  
dc.description.fil
Fil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/revista-medicina  
dc.conicet.rol
Autor  
dc.conicet.rol
Autor  
dc.conicet.rol
Autor  
dc.coverage
Nacional  
dc.type.subtype
Reunión  
dc.description.nombreEvento
LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología & 3er Congreso Franco Argentino de Inmunología; Reunión Anual 2022 de la Sociedad Argentina de Fisiología  
dc.date.evento
2022-11-16  
dc.description.ciudadEvento
Mar del Plata  
dc.description.paisEvento
Argentina  
dc.type.publicacion
Journal  
dc.description.institucionOrganizadora
Sociedad Argentina de Investigación Clínica  
dc.description.institucionOrganizadora
Sociedad Argentina de Inmunología  
dc.description.institucionOrganizadora
Sociedad Argentina de Fisiología  
dc.description.institucionOrganizadora
Sociedad Franco-Argentina de Inmunología  
dc.source.revista
Medicina (Buenos Aires)  
dc.date.eventoHasta
2022-11-19  
dc.type
Reunión