Artículo
Remote ischemic preconditioning prevents sarcolemmal-associated proteolysis by MMP-2 inhibition
Bin, Eliana Pamela; Zaobornyj, Tamara
; Garces, Mariana Soledad
; D'Anunzio, Verónica
; Buchholz, Bruno
; Marchini, Timoteo Oscar
; Evelson, Pablo Andrés
; Gelpi, Ricardo Jorge
; Donato, Pablo Martín
Fecha de publicación:
09/2023
Editorial:
Springer
Revista:
Molecular and Cellular Biochemistry
ISSN:
0300-8177
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The death of myocytes occurs through different pathways, but the rupture of the plasma membrane is the key point in the transition from reversible to irreversible injury. In the myocytes, three major groups of structural proteins that link the extracellular and intracellular milieus and confer structural stability to the cell membrane: the dystrophin-associated protein complex, the vinculin–integrin link, and the spectrin-based submembranous cytoskeleton. The objective was to determine if remote ischemic preconditioning (rIPC) preserves membrane-associated cytoskeletal proteins (dystrophin and β-dystroglycan) through the inhibition of metalloproteinase type 2 (MMP-2) activity. A second objective was to describe some of the intracellular signals of the rIPC, that modify mitochondrial function at the early reperfusion. Isolated rat hearts were subjected to 30 min of global ischemia and 120 min of reperfusion (I/R). rIPC was performed by 3 cycles of ischemia/reperfusion in the lower limb (rIPC). rIPC significantly decreased the infarct size, induced Akt/GSK-3 β phosphorylation and inhibition of the MPTP opening. rIPC improved mitochondrial function, increasing membrane potential, ATP production and respiratory control. I/R increased ONOO− production, which activates MMP-2. This enzyme degrades β-dystroglycan and dystrophin and collaborates to sarcolemmal disruption. rIPC attenuates the breakdown of β-dystroglycan and dystrophin through the inhibition of MMP-2 activity. Furthermore, we confirm that rIPC activates different intracellular pathway that involves the an Akt/Gsk3β and MPTP pore with preservation of mitochondrial function.
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(IBIMOL)
Articulos de INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR
Articulos de INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR
Citación
Bin, Eliana Pamela; Zaobornyj, Tamara; Garces, Mariana Soledad; D'Anunzio, Verónica; Buchholz, Bruno; et al.; Remote ischemic preconditioning prevents sarcolemmal-associated proteolysis by MMP-2 inhibition; Springer; Molecular and Cellular Biochemistry; 9-2023; 1-13
Compartir
Altmétricas