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dc.contributor.author
Knecht, Camila Ayelén  
dc.contributor.author
Hinkel, Maja  
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Mäusezahl, Ines  
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Kaster, Anne Kristin  
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Nivala, Jaime  
dc.contributor.author
Müller, Jochen A.  
dc.date.available
2024-02-21T13:15:13Z  
dc.date.issued
2023-06  
dc.identifier.citation
Knecht, Camila Ayelén; Hinkel, Maja; Mäusezahl, Ines; Kaster, Anne Kristin; Nivala, Jaime; et al.; Identification of Antibiotic Resistance Gene Hosts in Treatment Wetlands Using a Single-Cell Based High-Throughput Approach; MDPI; Water; 15; 13; 6-2023; 1-16  
dc.identifier.issn
2073-4441  
dc.identifier.uri
http://hdl.handle.net/11336/227759  
dc.description.abstract
Determining the prevalence of antimicrobial resistance (AMR) in non-clinical settings is vital for better management of the global AMR crisis. Untreated and even treated wastewaters are important sources that release AMR into the environment. Methodologically, it is difficult to generate a comprehensive in situ profile of antibiotic resistance gene hosts. Here, we used epicPCR (emulsion, paired isolation, and concatenation PCR) as a cultivation-independent method to reveal the host profiles of the AMR indicator genes intI1, sul1, sul2, and dfrA1 in two constructed wetlands treating municipal wastewater. Overall, the epicPCR analysis revealed a profile of AMR indicator gene hosts that is consistent with literature data from cultivation-based approaches. Most carriers of antibiotic resistance (AR) genes and likely of class 1 integrons belonged to the Gammaproteobateria, particularly the Burkholderiaceae and Rhodocyclaceae families, followed by members of the Campylobacterota, Desulfobacterota, and Firmicutes. The analysis also identified several novel hosts for the indicator genes widely distributed in the wetlands, including the genera Legionella and Ralstonia. Therefore, the application of epicPCR has produced an expanded insight into the in situ indicator gene host profile, while highlighting the role of the environment as a reservoir for AMR.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
MDPI  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/  
dc.subject
ANTIMICROBIAL RESISTANCE  
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CONSTRUCTED WETLAND  
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EPICPCR  
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SINGLE-CELL ANALYSIS  
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WASTEWATER  
dc.subject.classification
Bioremediación, Diagnóstico Biotecnológico en Gestión Medioambiental  
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Biotecnología del Medio Ambiente  
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INGENIERÍAS Y TECNOLOGÍAS  
dc.title
Identification of Antibiotic Resistance Gene Hosts in Treatment Wetlands Using a Single-Cell Based High-Throughput Approach  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2024-02-20T12:44:56Z  
dc.journal.volume
15  
dc.journal.number
13  
dc.journal.pagination
1-16  
dc.journal.pais
Suiza  
dc.description.fil
Fil: Knecht, Camila Ayelén. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina  
dc.description.fil
Fil: Hinkel, Maja. Helmholtz Centre for Environmental Research; Alemania  
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Fil: Mäusezahl, Ines. Helmholtz Centre for Environmental Research; Alemania  
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Fil: Kaster, Anne Kristin. Karlsruhe Institute of Technology; Alemania  
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Fil: Nivala, Jaime. Helmholtz Centre for Environmental Research; Alemania  
dc.description.fil
Fil: Müller, Jochen A.. Karlsruhe Institute of Technology; Alemania  
dc.journal.title
Water  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4441/15/13/2432  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3390/w15132432