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Artículo

Transferrin Enhances Neuronal Differentiation

Perez, Maria JuliaIcon ; Carden, Tomas RobertoIcon ; Dos Santos Claro, Paula AyelenIcon ; Silberstein Cuña, Susana IrisIcon ; Páez, Pablo Martin; Cheli, Verónica TeresitaIcon ; Correale, Jorge; Pasquini, Juana MariaIcon
Fecha de publicación: 01/2023
Editorial: SAGE Publications
Revista: ASN Neuro
ISSN: 1759-0914
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Neurociencias

Resumen

Although transferrin (Tf) is a glycoprotein best known for its role in iron delivery, iron-independent functions have also been reported. Here, we assessed apoTf (aTf) treatment effects on Neuro-2a (N2a) cells, a mouse neuroblastoma cell line which, once differentiated, shares many properties with neurons, including process outgrowth, expression of selective neuronal markers, and electrical activity. We first examined the binding of Tf to its receptor (TfR) in our model and verified that, like neurons, N2a cells can internalize Tf from the culture medium. Next, studies on neuronal developmental parameters showed that Tf increases N2a survival through a decrease in apoptosis. Additionally, Tf accelerated the morphological development of N2a cells by promoting neurite outgrowth. These pro-differentiating effects were also observed in primary cultures of mouse cortical neurons treated with aTf, as neurons matured at a higher rate than controls and showed a decrease in the expression of early neuronal markers. Further experiments in iron-enriched and iron-deficient media showed that Tf preserved its pro-differentiation properties in N2a cells, with results hinting at a modulatory role for iron. Moreover, N2a-microglia co-cultures revealed an increase in IL-10 upon aTf treatment, which may be thought to favor N2a differentiation. Taken together, these findings suggest that Tf reduces cell death and favors the neuronal differentiation process, thus making Tf a promising candidate to be used in regenerative strategies for neurodegenerative diseases.
Palabras clave: CYTOKINES , DIFFERENTIATION , N2A , NEURONS , TRANSFERRIN
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial 2.5 Unported (CC BY-NC 2.5)
Identificadores
URI: http://hdl.handle.net/11336/227671
DOI: http://dx.doi.org/10.1177/17590914231170703
Colecciones
Articulos(IBIOBA - MPSP)
Articulos de INST. D/INV.EN BIOMED.DE BS AS-CONICET-INST. PARTNER SOCIEDAD MAX PLANCK
Articulos(IQUIFIB)
Articulos de INST.DE QUIMICA Y FISICO-QUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Citación
Perez, Maria Julia; Carden, Tomas Roberto; Dos Santos Claro, Paula Ayelen; Silberstein Cuña, Susana Iris; Páez, Pablo Martin; et al.; Transferrin Enhances Neuronal Differentiation; SAGE Publications; ASN Neuro; 15; 1-2023; 1-20
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