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dc.contributor.author
Arias, Jose Luis  
dc.contributor.author
Funes, Samanta Celeste  
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Blas, Rodrigo  
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Callegari, Eduardo Alberto  
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Eliçabe, Ricardo Javier  
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Páez, María D.  
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Munarriz, Alicia  
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Pardo Hidalgo, Rodolfo  
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Tamashiro, Héctor  
dc.contributor.author
Di Genaro, Maria Silvia  
dc.date.available
2024-02-19T13:33:09Z  
dc.date.issued
2023-01  
dc.identifier.citation
Arias, Jose Luis; Funes, Samanta Celeste; Blas, Rodrigo; Callegari, Eduardo Alberto; Eliçabe, Ricardo Javier; et al.; S100A8 alarmin supports IL-6 and metalloproteinase-9 production by fibroblasts in the synovial microenvironment of peripheral spondyloar; Frontiers Media; Frontiers in Immunology; 13; 1-2023; 1-12  
dc.identifier.issn
1664-3224  
dc.identifier.uri
http://hdl.handle.net/11336/227404  
dc.description.abstract
Introduction: Spondyloarthritis (SpA) is a common autoinflammatory disease. S100A8/ S100A9 alarmin is strongly expressed in the synovial sublining layers of psoriatic arthritis. S100A8/ S100A9 is the most abundant protein in rheumatoid arthritis synovial fluid (SF) and has a key role in promoting IL-6 expression in fibroblast-like synoviocytes (FLS). The molecular mechanisms and the role of S100-alarmins in the synovial microenvironment of SpA have never been demonstrated. Methods and Results: Here, we confirm the effect of the synovial microenvironment of peripheral SpA on interleukin-6 (IL-6) and metalloproteinase (MMP)-9 production by FLS. MMP-9 expression and activity were detected, which were reduced in the presence of anti-IL-6R. Analyzing cell signaling mechanisms, we found that stimulation with IL-6 co-triggered MMP-9 and IL-10 secretion. MMP-9 secretion depended on JNK and p38 MAPKs, whereas IL-10 secretion was dependent on the JAK pathway as a potential feedback mechanism controlling IL-6-induced MMP-9 expression. Using a proteomic approach, we identified S100A8 in the peripheral SpA SF. This presence was confirmed by immunoblotting. S100A8 increased the IL-6 secretion via ERK and p38 MAPK pathways. Furthermore, anti-S100A8/A9 reduced both IL-6 and MMP-9 production induced by SpA SF in FLS. Discussion: Our data reveal a marked relationship between S100A8 alarmin with IL-6 and MMP-9 secretion by FLS in the real synovial microenvironment of peripheral SpA. These results identify a mechanism linking S100A8 to the pathogenesis of peripheral SpA.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Frontiers Media  
dc.rights
info:eu-repo/semantics/restrictedAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
FIBROBLAST-LIKE SYNOVIOCYTES  
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IL-6  
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MMP-9  
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S100A8  
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SPONDYLOARTHRITIS  
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SYNOVIAL FLUID  
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Inmunología  
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Medicina Básica  
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CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
S100A8 alarmin supports IL-6 and metalloproteinase-9 production by fibroblasts in the synovial microenvironment of peripheral spondyloar  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2024-02-19T10:19:24Z  
dc.journal.volume
13  
dc.journal.pagination
1-12  
dc.journal.pais
Suiza  
dc.journal.ciudad
Lausanne  
dc.description.fil
Fil: Arias, Jose Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina  
dc.description.fil
Fil: Funes, Samanta Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina  
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Fil: Blas, Rodrigo. Centro Medico Medici; Argentina  
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Fil: Callegari, Eduardo Alberto. University of South Dakota; Estados Unidos  
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Fil: Eliçabe, Ricardo Javier. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina  
dc.description.fil
Fil: Páez, María D.. University of South Dakota; Estados Unidos  
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Fil: Munarriz, Alicia. Instituto Privado Cenyr; Argentina  
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Fil: Pardo Hidalgo, Rodolfo. Centro de Rehabilitación Médica; Argentina  
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Fil: Tamashiro, Héctor. Centro Médico Bolivar; Argentina  
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Fil: Di Genaro, Maria Silvia. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina  
dc.journal.title
Frontiers in Immunology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fimmu.2022.1077914/full  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3389/fimmu.2022.1077914