Artículo
Two-Step Preparation of Protein-Decorated Biohybrid Quantum Dot Nanoparticles for Cellular Uptake
Traverso, Agata Noelia; Fragale, David José; Viale, Diego Luis
; Garate, Octavio Federico; Torres, Pablo Sebastian
; Valverde, Gastón; Berra, Alejandro; Torbidoni, Ana Vanesa
; Yakisich, Juan Sebastián; Grasselli, Mariano
; Radrizzani, Martín
Fecha de publicación:
06/2023
Editorial:
MDPI
Revista:
Pharmaceutics
ISSN:
1999-4923
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Decoration of nanoparticles with specific molecules such as antibodies, peptides, and proteins that preserve their biological properties is essential for the recognition and internalization of their specific target cells. Inefficient preparation of such decorated nanoparticles leads to nonspecific interactions diverting them from their desired target. We report a simple two-step procedure for the preparation of biohybrid nanoparticles containing a core of hydrophobic quantum dots coated with a multilayer of human serum albumin. These nanoparticles were prepared by ultra-sonication, crosslinked using glutaraldehyde, and decorated with proteins such as human serum albumin or human transferrin in their native conformations. These nanoparticles were homogeneous in size (20–30 nm), retained the fluorescent properties of quantum dots, and did not show a “corona effect” in the presence of serum. The uptake of transferrin-decorated quantum dot nanoparticles was observed in A549 lung cancer and SH-SY5Y neuroblastoma cells but not in non-cancerous 16HB14o- or retinoic acid dopaminergic neurons differentiated SH-SY5Y cells. Furthermore, digitoxin-loaded transferrin-decorated nanoparticles decreased the number of A549 cells without effect on 16HB14o-. Finally, we analyzed the in vivo uptake of these biohybrids by murine retinal cells, demonstrating their capacity to selectively target and deliver into specific cell types with excellent traceability.
Palabras clave:
ALBUMIN
,
DRUG DELIVERY
,
ENDOCYTOSIS
,
NANOPARTICLES
,
TRANSFERRIN
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Licencia
Identificadores
Colecciones
Articulos (ITECA)
Articulos de INSTITUTO DE TECNOLOGÍAS EMERGENTES Y CIENCIAS APLICADAS
Articulos de INSTITUTO DE TECNOLOGÍAS EMERGENTES Y CIENCIAS APLICADAS
Articulos(IMBICE)
Articulos de INST.MULTIDISCIPL.DE BIOLOGIA CELULAR (I)
Articulos de INST.MULTIDISCIPL.DE BIOLOGIA CELULAR (I)
Citación
Traverso, Agata Noelia; Fragale, David José; Viale, Diego Luis; Garate, Octavio Federico; Torres, Pablo Sebastian; et al.; Two-Step Preparation of Protein-Decorated Biohybrid Quantum Dot Nanoparticles for Cellular Uptake; MDPI; Pharmaceutics; 15; 6; 6-2023; 1-19
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