Construction of electroactive polyamine-enzyme assemblies nondependent on the electrical charge

Abstract

The assembly of polyelectrolytes and biomacromolecules (proteins, DNA) has attracted great attention in several fields related to medicine, molecular biology, and clinical chemistry. Here, a quick and easy multicomponent self-assembly is used to unite two species with the same charge to form an electroactive polyamine-enzyme assembly. Phosphate ions are added as a crosslinker agent to create a stable structure. Osmium-derivatized polyallylamine is combined with horseradish peroxidase (pI = 8.6) or PQQ-glucose dehydrogenase (pI = 9.5) on graphite electrodes. The assemblies are built by layer-by-layer or one-pot deposition. In both cases, the electrocatalytical signals observed are comparable to the state-of-the-art results. The proposed construction method represents a new alternative for the assembly of polyamines with proteins independently of their charge.