Mostrar el registro sencillo del ítem
dc.contributor.author
Guillen, Laura Cristina
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.contributor.author
Vodanovich Florencia
dc.contributor.author
Mauriño, Eduardo
dc.contributor.author
Hwang, H.
dc.contributor.author
Bai, Julio
dc.contributor.author
Cherñavsky, Alejandra Claudia
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.date.available
2024-02-15T11:10:30Z
dc.date.issued
2011
dc.identifier.citation
A differential expression of SLAM (Signaling and Activation of Lymphocytes molecule) is induced by gliadin on antigen presenting cells in patients with active Celiac Disease; Digestive Disease Week; Chicago; Estados Unidos; 2011; 1-1
dc.identifier.uri
http://hdl.handle.net/11336/226963
dc.description.abstract
Background: Monocytes (Mo) recruited into the intestinal mucosa are source of macrophages and dendritic cells (DC). Under inflammatory conditions, lymphocytes (L) are co recruited and direct (cell to cell contact) or indirectly (by soluble factors) influence Mo outcome. In vitro interactions between blood cells and soluble factors produced by them may influence Mo activation and differentiation into DC. SLAM (Signaling and Activation of Lymphocytes molecule) and OX40 ligand (OX40-L) co-stimulatory molecules expressed on antigen presenting cells (APC) are involved in the interaction with activated L. Aim: to examine the influence of gliadin on APC in celiac patients (Ce) and healthy controls (Co) by measuring SLAM and OX40-L expression. Materials: 15 adults (> 18y) diagnosed as celiacs and 15 age-matched controls were enroled. Mo activation: Peripheral blood mononuclear cells (PBMC) were isolated, cultured at 106 cells/ml for 24h with complete RPMI-1640, gliadin (25 ug/ml), urea (2M) and LPS ( 1ug/ul), stained with conjugated mAb anti -CD14, -OX40-L and -SLAM, and analyzed by flow cytometry. (FC). Interleukin (IL) -8 was determined by ELISA in supernatants of Mo previously isolated by Percoll gradients. Mo-derive DC: Adherent PBMC were incubated with GM-CSF (800U/ml) and IL-4 (10ng/ml) for 5 days, induced to mature in the presence of gliadin (75ug/ml) for 2 days and analyzed for the expression of surface CD11c, HLA-DR, CD86, SLAM and OX40-L by FC. Median stimulation indexes (Ĩ) were defined as [gliadin-stimulated Mo or DC / basal Mo or DC] to measure IL-8 production and % of double positive Mo (CD14+ plus SLAM/OX40L) and DC (CD11c+ plus SLAM/OX40-L/ CD86/ HLADR). The Ĩ were compared by Mann-Whitney test. Results: IL-8 was spontaneously released by Mo from Co and Ce (p=NS) and further stimulated by gliadin (Ĩ IL-8 : 2.70 ± 0.70 vs. 1.40 ± 0.05, p=0,028; Ce vs. Co). SLAM expression was higher in gliadin-stimulated Mo (Ĩ% CD14+SLAM+: 10.60 ± 1.40 vs. 6.30 ± 0.90, p=0.009; Ce vs. Co) and DC (Ĩ CD11c+SLAM+: 6.30 ±1.10 vs.3.20 ±1.20, p= 0.028; Ce vs. Co). After gliadin treatment, similar Ĩ% CD14+CD86 and Ĩ% CD14+HLA-DR were calculated in mature DC from Ce and Co (p=NS). After gliadin treatments, OX40-L was similarly expressed on Mo and DC from Ce and Co (p=NS). Conclusions: Although we used both a well-defined cytokine cocktail and gliadin as definite inductors for Mo activation, differentiation and DC maduration, SLAM expression was enhanced on APC from Ce patients. Our results suggest that besides the presence of gliadin, a particular peripheral microenvironment might contribute to the amplification and specialization of the adaptive immune response in celiac disease. The functional involvement of SLAM is currently under investigation.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
SLAM
dc.subject
GLIADIN
dc.subject
ACTIVE CELIAC DISEASE
dc.subject.classification
Otras Ciencias de la Salud
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
Ciencias de la Salud
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.title
A differential expression of SLAM (Signaling and Activation of Lymphocytes molecule) is induced by gliadin on antigen presenting cells in patients with active Celiac Disease
dc.type
info:eu-repo/semantics/publishedVersion
dc.type
info:eu-repo/semantics/conferenceObject
dc.type
info:ar-repo/semantics/documento de conferencia
dc.date.updated
2023-09-25T15:00:22Z
dc.identifier.eissn
0016-5085
dc.journal.volume
140
dc.journal.pagination
1-1
dc.journal.pais
Estados Unidos
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.journal.ciudad
Chicago
dc.description.fil
Fil: Guillen, Laura Cristina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clinicas Gral.san Martin. Laboratorio Nacional de Investigaciones y Servicios; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Vodanovich Florencia. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clinicas Gral.san Martin. Laboratorio Nacional de Investigaciones y Servicios; Argentina
dc.description.fil
Fil: Mauriño, Eduardo. Gobierno de la Ciudad de Buenos Aires. Hospital de Gastroenterología "Dr. Carlos B. Udaondo"; Argentina
dc.description.fil
Fil: Hwang, H.. Gobierno de la Ciudad de Buenos Aires. Hospital de Gastroenterología "Dr. Carlos B. Udaondo"; Argentina
dc.description.fil
Fil: Bai, Julio. Gobierno de la Ciudad de Buenos Aires. Hospital de Gastroenterología "Dr. Carlos B. Udaondo"; Argentina
dc.description.fil
Fil: Cherñavsky, Alejandra Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.gastrojournal.org/article/S0016-5085(11)62664-4/fulltext
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/S0016-5085(11)62664-4
dc.conicet.rol
Autor
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.conicet.rol
Autor
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.conicet.rol
Autor
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.conicet.rol
Autor
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.conicet.rol
Autor
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.conicet.rol
Autor
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.coverage
Internacional
dc.type.subtype
Congreso
dc.description.nombreEvento
Digestive Disease Week
dc.date.evento
2011-05-01
dc.description.ciudadEvento
Chicago
dc.description.paisEvento
Estados Unidos
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.type.publicacion
Journal
dc.description.institucionOrganizadora
American Gastroenterogical Association
dc.source.libro
Libro de Resúmenes
dc.source.revista
Gastroenterology
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.date.eventoHasta
2011-05-01
dc.type
Congreso
Archivos asociados