Artículo
Biointerfacial behavior of stallion spermatozoa adhered to hydrogel surfaces: Impact of the hydrogel chemical composition and the culture medium
Ebel Barrera, Francisca Andrea
; Liaudat, Ana Cecilia
; Blois, Damián Andrés
; Capella, Virginia
; Broglia, Martin Federico
; Barbero, César Alfredo
; Rodríguez, Nancy; Bosch, Pablo
; Rivarola, Claudia Rosana
Fecha de publicación:
11/2023
Editorial:
Elsevier Science
Revista:
Colloids and Surfaces B: Biointerfaces
ISSN:
0927-7765
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Novel soft materials based on hydrogel are proposed to enhance the selection of high-quality stallion sperm based on their adhesion capacity. The hydrogel surfaces are derived from polyacrylamide (PAAm), which is copolymerized with neutral and ionic co-monomers to modify the interfacial properties. The hydrogels undergo characterization through FTIR spectroscopy, assessment of swelling capacity, and wettability under various experimental conditions. Sperm adhesion capacity on the hydrogels is examined through several parameters including the percentage of bound sperm (%Sp) to hydrogels, tail oscillation intensity and flagellar movement. The biointerfacial properties of sperm-hydrogel systems vary based on the chemical composition of hydrogel as well as the components present in the culture medium. High %Sp and excellent metabolic activity of the spermatozoa are observed on hydrogel surfaces that possess moderate hydrophilicity. Specifically, a cationic hydrogel in BGM3 culture medium and a neutral surface in BGM3 medium supplemented with BSA exhibit favorable outcomes. Scanning Electron Microscopy (SEM) reveals the normal morphology of the head and tail in spermatozoa adhered to the hydrogel. Therefore, these hydrogel surfaces are potential materials for selecting stallion sperm with high quality, and their application could be extended to the study of other mammalian reproductive cells.
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Colecciones
Articulos (IITEMA)
Articulos de INSTITUTO DE INVESTIGACIONES EN TECNOLOGIAS ENERGETICAS Y MATERIALES AVANZADOS
Articulos de INSTITUTO DE INVESTIGACIONES EN TECNOLOGIAS ENERGETICAS Y MATERIALES AVANZADOS
Articulos (INBIAS)
Articulos de INSTITUTO DE BIOTECNOLOGIA AMBIENTAL Y SALUD
Articulos de INSTITUTO DE BIOTECNOLOGIA AMBIENTAL Y SALUD
Citación
Ebel Barrera, Francisca Andrea; Liaudat, Ana Cecilia; Blois, Damián Andrés; Capella, Virginia; Broglia, Martin Federico; et al.; Biointerfacial behavior of stallion spermatozoa adhered to hydrogel surfaces: Impact of the hydrogel chemical composition and the culture medium; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 231; 11-2023; 1-9
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