Artículo
Ultrastructural and molecular evidence of macroautophagy in functioning PitNETs and experimental pituitary tumors
Cecenarro, Laura Anahi; Gabriela Deisi, Moyano Crespo; Guido, Carolina Beatriz
; Pérez, Pablo Aníbal
; Faure, Erica Elizabeth; Picech, Florencia
; Rossetto, Sofía Victoria
; de Battista, Juan Carlos
; Gutiérrez, Silvina
; Torres, Alicia Ines
; Petiti, Juan Pablo
; Mukdsi, Jorge Humberto
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Fecha de publicación:
01/2023
Editorial:
Karger
Revista:
Neuroendocrinology
ISSN:
0028-3835
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Introduction: Macroautophagy is a lysosome-mediated degradation process that controls the quality of cytoplasmic components and organelles, with its regulation depending on autophagy-related proteins (Atg) and with Beclin1/Atg6 and microtubule-associated protein light chain 3 (LC3/Atg8) being key players in the mammalian autophagy. As reports on this mechanism in the field of pituitary neuropathology and neuroendocrinology are scarce, our study analyzed the ultrastructural signs of macroautophagy and the expression of Beclin1 and LC3 proteins in human functioning PitNETs and in experimental pituitary tumors. Methods: A group of humans functioning PitNETs and an experimental lactotroph model in rats of the F344 strain stimulated with estradiol benzoate (BE) were used. Ultrastructural and molecular evidence of the macroautophagic process was evaluated using different techniques. Results: In functioning PitNETs cohort, 60% exhibited evidence of macroautophagy, with a significant difference found for Beclin1 and LC3 between macro- and micro-PitNETs (p < 0.05). In the experimental model, the expression of both Beclin1 and LC3 proteins was immunopositive in normal and tumoral glands when analyzed by immunofluorescence, Western blot, and immunohistochemistry. In the experimental model, protein expression was associated with increased glandular size and weight. Conclusions: Our study revealed evidence of macroautophagy at the pituitary level and the important role of Beclin1 and LC3 in the progression of functioning PitNETs, implying that this mechanism participate in regulating pituitary cell growth.
Palabras clave:
MACROATOPHAGY
,
PITNET
,
LC3 AND BECLIN
,
ULTRASTRUCTURE
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Identificadores
Colecciones
Articulos(INICSA)
Articulos de INSTITUTO DE INVESTIGACIONES EN CIENCIAS DE LA SALUD
Articulos de INSTITUTO DE INVESTIGACIONES EN CIENCIAS DE LA SALUD
Citación
Cecenarro, Laura Anahi; Gabriela Deisi, Moyano Crespo; Guido, Carolina Beatriz; Pérez, Pablo Aníbal; Faure, Erica Elizabeth; et al.; Ultrastructural and molecular evidence of macroautophagy in functioning PitNETs and experimental pituitary tumors; Karger; Neuroendocrinology; 113; 7; 1-2023; 705-718
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